Functionalized silicon compounds and methods for their synthesis and use
First Claim
1. A method of functionalizing a surface, comprising a covalently attaching to the surface a functionalized silicon compound having a structure of Formula 3:
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wherein R1 and R2 are independently selected from the group consisting of alkoxy or halide, and R3 is selected from the group consisting of alkoxy, halide and alkyl;
wherein L1, L2, and L3 are independently —
(CH2)n—
, wherein n is 2-10; and
wherein A1 and A2 are independently a moiety comprising one or more derivatizable functional groups or modified forms thereof.
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Abstract
Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.
64 Citations
9 Claims
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1. A method of functionalizing a surface, comprising a covalently attaching to the surface a functionalized silicon compound having a structure of Formula 3:
-
wherein R1 and R2 are independently selected from the group consisting of alkoxy or halide, and R3 is selected from the group consisting of alkoxy, halide and alkyl;
wherein L1, L2, and L3 are independently —
(CH2)n—
, wherein n is 2-10; and
wherein A1 and A2 are independently a moiety comprising one or more derivatizable functional groups or modified forms thereof. - View Dependent Claims (2, 3, 4, 5, 7, 8, 9)
forming an array of nucleic acids covalently attached to the functionalized silicon compounds on the surface.
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3. The method of claim 1, wherein the method comprising covalently attaching to the surface a functionalized silicon compound having a structure of Formula 3:
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wherein R1 and R2 are independently alkoxy or halide, and R3 is selected from the group consisting of alkoxy, halide and alkyl;
wherein L1, L2, and L3 are independently —
(CH2)n—
, wherein n is 2 to 10 ; and
wherein A1 and A2 are independently H or a moiety comprising one or more derivatizable functional groups selected from the group consisting of hydroxyl, amino, amido, carboxyl, thio, halo, and sulfonate.
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4. The method of claim 3, wherein the one or more derivatizable functional groups are hydroxyl groups, activated hydroxyl groups or protected hydroxyl groups.
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5. The method of claim 3, wherein A1 and A2 each comprise a plurality of derivatizable functional groups.
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7. The method of claim 1, wherein the surface is the surface of a substrate comprising silica.
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8. The method of claim 3, wherein the surface is the surface of a substrate comprising silica.
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9. The method of claim 1, wherein the substrate is in a form selected from the group consisting of particles, films and chips.
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6. A method of functionalizing a surface comprising covalently attaching a functionalized silicon compound to the surface, wherein the silicon compound is a compound having a structure of Formula V:
Specification