Systems and methods for optical examination of samples

• US 6,411,838 B1
• Filed: 12/22/1999
• Issued: 06/25/2002
• Est. Priority Date: 12/23/1998
• Status: Expired due to Term

• Current Assignee: Luma Imaging Corp. (SpectraScience, Inc.)
• Original Assignee: MediSpectra, Inc. (SpectraScience, Inc.)
• Inventors: Modell, Mark, Nordstrom, Robert, Zelenchuk, Alexander
• Primary Examiner(s): Lateef, Marvin M.
• Assistant Examiner(s): QADERI, RUNA S
• Application Number:
  US09/470,071
• Time in Patent Office: 916 Days
• Field of Search: 600/407, 600/423, 600/425, 600/473, 600/476, 600/478
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0068 Confocal scanning

Abstract

The present invention relates to systems and methods for examining a sample using a substantially monostatic, substantially confocal optical system comprising transmitting optics that focus an illuminating light upon the sample and receiving optics that collect light emitted from the sample following illumination thereof. In certain embodiments, the receiving optics may be arranged circumferentially around the light path traversed by the illuminating light. In certain embodiments, video apparatus may be included to produce images or to align the system in proximity to the target tissue. The systems and methods of the present invention may be directed towards the examination of a body tissue to provide a medical diagnosis.

Claims

1. A system for examining a sample, comprising:

an optical probe, comprising a plurality of optical fibers and capable of illuminating the sample;

a substantially monostatic, substantially confocal optical system comprising transmitting optics that focus an illuminating light upon the sample, and receiving optics, having components separated and distinct from the transmitting optics, that collect light emitted from the sample following illumination thereof; and

a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the receiving optics.

2. The system of claim 1, wherein the optical system further comprises reflective optical components.

3. The system of claim 1, wherein the optical system further comprises refractive optical components.

4. The system of claim 1, wherein the optical system brings illuminating light to a focus point at a location in the sample and wherein the optical system collects light emitted from that same said location in the sample as a result of illumination with the illuminating light.

5. The system of claim 1, wherein the receiving optics are arranged circumferentially around a light path for the illuminating light.

6. The system of claim 1, further comprising a scanner that directs the illuminating light towards the sample by illuminating individual optical fibers in a preselected pattern.

7. The system of claim 6, wherein the preselected pattern comprises a rectilinear array pattern.

8. The system of claim 6, wherein the preselected pattern comprises a hexagonal pattern.

9. The system of claim 1, wherein the illuminating light comprises a laser and wherein the light emitted from the sample comprises fluorescence.

10. The system of claim 1, wherein the illuminating light is provided by a broadband source and the emitted light comprises elastic backscattered light.

11. The system of claim 1, wherein the illuminating light is provided by a pulsed laser and the emitted light comprises Raman scattered light.

12. The system of claim 1, wherein the illuminating light comprises a nitrogen laser.

13. The system of claim 1, wherein the illuminating light comprises a Nd:

YAG laser, tripled in output frequency to a wavelength of 355 nm.

14. The system of claim 1, wherein the illuminating light is a broadband light provided by a xenon lamp.

15. A system for determining a characteristic of a sample, comprising:

an optical probe for substantially monostatic, substantially confocal examination of the sample;

an optics system comprising transmitting optics that focus an illuminating light on the sample, receiving optics, having components separated and distinct from the transmitting optics, that collect light emitted from the sample, and a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the receiving optics;

a measuring system that produces quantitative data related to the light emitted from the sample; and

a processor that processes the quantitative data to determine the characteristics of the sample.

16. The system of claim 15, further comprising a video system, wherein the video system transmits to a display an image of a surface of the sample.

17. The system of claim 15, further comprising a position sensor whereby a position of the optical probe in relation to the sample may be determined.

18. The system of claim 17, wherein the position sensor comprises a focusing image projected upon the surface of the sample, whereby the position of the optical probe in relation to the sample is determined by evaluating clarity of focus for the focusing image.

19. An optical probe system for substantially monostatic, substantially confocal examination of a sample, comprising:

an optical probe that directs an illuminating light towards the sample and that collects light emitted from the sample;

a light source providing the illuminating light;

transmitting optics focusing the illuminating light on the sample;

collecting optics, having components separated and distinct from the transmitting optics, arranged substantially as an annulus surrounding a light path for the illuminating light, wherein the collecting optics receive light emitted from the sample;

a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the collecting optics; and

a connecting circuit that transmits electromagnetic energy related to light emitted from the sample to a processor for further processing.

20. The optical probe system of claim 19, further comprising a scanning system that sequentially illuminates a plurality of optical fibers, thereby passing a point of illumination over a surface of the sample in a preselected pattern.

21. The optical probe system of claim 19, further comprising a video channel for viewing a surface of the sample and for determining a location of the probe relative to the sample.

22. The optical probe system of claim 21, further comprising a video camera dimensionally adapted for mounting on the optical probe.

23. The optical probe system of claim 21, wherein the video channel shares an optical path of the illuminating light in the optical probe.

24. A method for examining a sample, comprising:

providing a substantially monostatic, substantially confocal optical probe comprising transmitting optics and collecting optics, having components separated and distinct from the transmitting optics, wherein the collecting optics are disposed around a circumference of a light path for transmitting an illuminating light toward the sample, and a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the collecting optics;

determining an optimal position for positioning the probe in relation to the sample;

positioning the probe in the optimal position in relation to the sample;

illuminating the sample with a light beam transmitted through the transmitting optics; and

collecting light emitted from the sample as a result of its illumination.

25. The method of claim 24, further comprising processing electromagnetic energy related to the light to derive data related to the light.

26. The method of claim 25, further comprising creating a graphic image to represent the data related to the light.

27. The method of claim 24, wherein a video apparatus directs towards the sample a focusing image employed in determining the optimal position for the probe in relation to the sample.

28. A method for diagnosing a medical condition, comprising:

providing a substantially monostatic, substantially confocal optical probe comprising transmitting optics and collecting optics, having components separated and distinct from the transmitting optics, wherein the collecting optics are disposed around a circumference of a light path for transmitting an illuminating light toward a body tissue, and a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the collecting optics;

illuminating the body tissue;

collecting light emitted from the body tissue;

measuring a set of data related to the light collected from the body tissue; and

diagnosing from the set of data the medical condition.

29. The method of claim 28, further comprising processing the set of data with a processor.

30. The method of claim 28, further comprising creating a graphical image that represents the set of data.

31. A system for examining a body tissue, comprising:

an optical probe that directs an illuminating light towards the body tissue and that collects light emitted from the body tissue;

a substantially monostatic, substantially confocal optical system comprising transmitting optics that focus the illuminating light on the body tissue and receiving optics, having components separated and distinct from the transmitting optics, and a barrier element adapted to inhibit scattered light from the illuminating light from entering a portion of the receiving optics, wherein the receiving optics collect light emitted from the body tissue; and

a measuring system that produces quantitative data related to the light emitted from the body tissue.

32. The system of claim 31, wherein the body tissue is a cervix uteri.