Zero dead time architecture for flow cytometer

• US 6,411,904 B1
• Filed: 05/13/1999
• Issued: 06/25/2002
• Est. Priority Date: 05/14/1998
• Status: Expired due to Term

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Inventors: Chandler, Van S.
• Primary Examiner(s): Hoff, Marc S.
• Assistant Examiner(s): Barbee, Manuel L.
• Application Number:
  US09/310,755
• Time in Patent Office: 1,139 Days
• Field of Search: 702/19-22, 702/30-32, 702/45, 702/80, 702/100, 702/127, 702/128, 702/179, 702/180, 702/183, 702/187-189, 702/193, 700/72, 700/73, 710/52, 710/56
• US Class Current: 702/21
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1477 Multiparameters
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G01N 2021/4707 Forward scatter; Low angle scatter
• CPC Class Codes: G01N 2021/4726 Detecting scatter at 90°
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters

Abstract

A method and apparatus for providing a zero dead time for flow analyzers, flow cytometers, and other measurement devices. A circular buffer is used to store data from a flow analyzer in a plurality of data storage areas, until it is convenient to implement more time consuming data processing procedures. User specified parameters, including sampling rate and/or sampling period, size and number of data storage areas, size of the circular or other buffer, signal-to-noise threshold, order of processing when a plurality of Digital Signal Processors (DSPs) is used, and fixed trailing distance, are used to provide flexible and convenient operation by a user. The probability of missing a rare event occurring within the laser beam or other light beam of a flow analyzer or other measurement device is reduced to zero. Functional equivalents may be used in place of the circular buffer, for example, First In-First Out buffers which route data to a cache for potential re-reading and/or reprocessing or additional processing of data, cascading buffers, etc. Circular and functionally equivalent buffers permit sophisticated data processing in which preceding data (for example, data not meeting the S/N threshold) subsequently are re-analyzed for the possibility that the data are part of a developing event peak, such as a developing shoulder thereof, or part of a declining peak. In addition, logic and algorithms to permit re-examination and potential reprocessing of “old” subthreshold data is utilized to improve the S/N ratio of an event peak.

Claims

1. A data collection and measurement device collecting data with substantially zero dead time, comprising:

a) a circular buffer comprising;

a plurality of data storage areas that are linked to receive and consecutively store incoming data from a plurality of successive sampling periods for at least one event of a measurement device, where each data storage area is configured to receive and store data from one sampling period, and the data storage areas are linked in an order that provides storage for data from a next-in-time sampling period into the next-in-order data storage area;

a First pointer; and

a Second pointer; and

b) at least one Digital Signal Processor (DSP), where the First Pointer directs receipt and storage of data of the next-in-time sampling period into the next-in-order data storage area, and, once all data storage areas contain data, directs receipt of data of the next-in-time sampling period, and storage by overwriting therewith data in the next-in-order data storage area, the Second Pointer is directed to the data storage area that stores data from a sample period preceding a current time of data receipt and storage by a fixed trailing distance as a referenced to the First Pointer, and directs one of the at least one DSPs to read and process data in the data storage area to which the Second Pointer is directed, and wherein once the next-in-time data have been stored, the First Pointer advances to the next-in-order data storage area, and once data in the data storage area to which the Second Pointer is directed have been processed, the Second Pointer advances to the next-in-order data storage area,

wherein said collection and measurement apparatus collects and processes data from the at least one event, and where the fixed trailing distance equals at least a longest possible time for an event.

2. The data collection and measurement device of claim 1, where the fixed trailing distance is about 10 to about 100 milliseconds.

3. The data collection and measurement device of claim 1, where the fixed trailing distance is user specified.

4. The data collection and measurement device claim 1, where the sampling periods are of a fixed length of time, and the fixed length of time is user specified.

5. The data collection and measurement device of claim 4, where the sampling periods are each about one millionth of a second.

6. The data collection and measurement device of claim 4, where the sampling periods are each less than one millionth of a second.

7. The data collection and measurement device of claim 1, where the number of data storage areas is user specified.

8. The data collection and measurement device of claim 7, where the number of data storage areas is at least one thousand.

9. The data collection and measurement device of claim 1, where each data storage area has a size, and the size is user specified.

10. The data collection and measurement device of claim 9, where the size of each data storage area is from about 12 bits to about 16 bits.

11. The data collection and measurement device of claim 9, where the size of each data storage area is greater than 16 bits.

12. The data collection and measurement device of claim 9, where the size of each data storage area is less than 12 bits.

13. The data collection and measurement device of claim 1, further comprising an order of data processing by DSPs when a plurality of DSPs is employed.

14. The data collection and measurement device of claim 13, where the order of data processing by DSPs is user specified.

15. The data collection and measurement device of claim 1, further comprising a signal-to-noise ratio threshold that serves as a criterion as to whether data are processed by the at least one DSP.

16. The data collection and measurement device of claim 15, where the signal-to-noise ratio threshold is user specified.

17. The data collection and measurement device of claim 15, further comprising logic to re-examine and reprocess previous data that do not meet the signal-to-noise ratio threshold.

18. A flow analyser collecting data with substantially zero dead time for at least one event, comprising:

an energy beam in communication with a particle to be measured, said particle in an examination zone for a predetermined period of time;

a sensor configured to measure a particle signal emitted as a result of said energy beam being communicated to said particle;

a circular buffer coupled to said sensor configured to read and store a digital signal representing data associated with said particle signal, wherein said circular buffer comprises,

a plurality of data storage areas that are linked to receive and consecutively store incoming data from a plurality of successive sampling periods for at least one event of said sensor, where each data storage area is configured to receive and store data from one sampling period, and the data storage areas are linked in an order that provides storage for data from a next-in-time sampling period into the next-in-order data storage area data storage area; and

a first pointer; and

a second pointer; and

at least one Digital Signal Processor (DSP), where the First Pointer directs receipt and storage of data of the next-in-time sampling period into the next-in-order data storage area, and, once all data storage areas contain data, directs receipt of data of the next-in-time sampling period, and storage by overwriting therewith data in the next-in-order data storage area, the Second Pointer is directed to the data storage area that stores data from a sample period preceding a current time of data receipt and storage by a fixed trailing distance as a referenced to the First Pointer, and directs one of the at least one DSPs to read and process data in the data storage area to which the Second Pointer is directed, and wherein once the next-in-time data have been stored, the First Pointer advances to the next-in-order data storage area, and once data in the data storage area to which the Second Pointer is directed have been processed, the Second Pointer advances to the next-in-order data storage area, wherein said collection and measurement apparatus collects and processes data from the at least one event, and where the fixed trailing distance equals at least a longest possible time for an event.

19. The flow analyser of claim 18, further comprising a data conditioning device coupled to said sensor for conditioning said particle signal by at least one conditioning method selected from the group consisting of filtering and amplification.

20. The flow analyser of claim 18, wherein said flow analyser comprises a flow cytometer.

21. The flow analyser of claim 18, wherein said energy beam comprises a laser beam.

22. The flow analyser of claim 18, wherein said particle comprises a cell.

23. The flow analyser of claim 18, wherein said particle is transferred to said examination zone by hydrodynamic focusing.