System and device for determining particle characteristics

• US 6,412,359 B1
• Filed: 06/30/2000
• Issued: 07/02/2002
• Est. Priority Date: 02/06/1998
• Status: Expired due to Term

• Current Assignee: Cleveland Clinic Foundation, Ohio State Innovation Foundation (Ohio State University)
• Original Assignee: Cleveland Clinic Foundation, Ohio State University
• Inventors: Zborowski, Maciej, Chalmers, Jeff, Moore, Lee Robert
• Primary Examiner(s): RAEVIS, ROBERT R
• Application Number:
  US09/607,702
• Time in Patent Office: 732 Days
• Field of Search: 738/655, 73/616.9, 324/71.4, 324/201, 324/228, 348/142
• US Class Current: 73/865.5
• CPC Class Codes: G01N 15/10 Investigating individual particles
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1027 Determining speed or velocity of a particle
• CPC Class Codes: G01N 2015/1028 Sorting particles
• CPC Class Codes: G01N 27/447 using electrophoresis
• CPC Class Codes: G01P 5/18 by measuring the time taken to traverse a fixed distance

Abstract

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.

Claims

1. A device for determining one or more particle physical characteristics, the device comprising:

(a) a force field device for subjecting at least one particle to at least one force field, the force field device comprising;

(1) a first force field producing assembly comprising;

(i) a first force field producing device; and

(ii) a first pole piece for concentrating the force field flux produced by the first force field producing device;

(2) a second force field producing assembly comprising;

(i) a second force field producing device; and

(ii) a second pole piece for concentrating the force field flux produced by the second force field producing device;

(3) the first and second pole pieces each comprising a flux concentrating portion having a curved end portion for producing a region of space having a substantially constant force field and wherein the flux concentrating portions are displaced substantially opposite each other with an inter-polar air gap therebetween and wherein the curved end portions comprise;

(i) a first distal end having a substantially curved surface;

(ii) a second distal end having a substantially curved surface;

(iii) wherein the first and second distal ends are configured so as to be contiguous with each other; and

(iv) wherein the substantially curved surface of the first distal end comprises a portion having a substantially constant radius and wherein the radius is in the range of approximately 3 mm; and

(v) wherein the substantially curved surface of the second distal end comprises a portion which is substantially hyperbolic and wherein the hyperbolic portion is approximately defined as y(x)=9.544/x2−

12.719, wherein x and y are Cartesian coordinates with the origin placed at the intersection of the plane of symmetry separating the pole pieces and a plane tangent to the radial portion of the distal ends of the pole pieces;

(b) a substantially transparent flow channel positioned at least partially within the region of space having a substantially constant force magnitude and direction and for allowing the introduction of at least one particle; and

(c) a computer system for gathering and analyzing data associated with the at least one particle.

2. The device of claim 1 wherein the first and second pole pieces further comprise substantially planar top and bottom surfaces, wherein the substantially planar bottom surface of the first pole piece is at least partly in physical contact with the first force field producing device and the substantially planar bottom surface of the second pole piece is at least partly in physical contact with the second force field producing device.

3. The device of claim 2 further comprising a force field control device for controlling the magnitude of the force field.

4. The device of claim 2 wherein the flux concentrating portion of the first force field producing assembly projects from the first force field producing device and the flux concentrating portion of the second force field producing assembly projects from the second force field producing device.

5. The device of claim 1 wherein the first and second force field producing devices are magnets.

6. The device of claim 1 wherein the first and second force field producing devices are electromagnets.

7. The device of claim 1 wherein the first and second force field producing devices are electric force field producing devices.

8. The device of claim 1 further comprising at least one utility space positioned near the region having a substantially constant force fielded.

9. The method of claim 1 wherein the particle is selected from a group consisting of:

cells, cell organelles, platelets, inorganic, organic, biological, and polymeric particles which are optically visible.

10. A device for determining one or more particle physical characteristics, the device comprising:

(a) a force field device for subjecting at least one particle to at least one force field, the force field device comprising;

(1) a base;

(2) first and second magnets coupled to the base for generating a magnetic flux;

(3) first and second pole pieces coupled to the first and second magnets;

the first and second pole pieces comprising a substantially planar shape having a magnetic flux concentrating portion; and

(4) the first and second pole pieces further configured to provide an air gap and a utility space for accommodating data collection equipment, wherein the air gap at least partially forms a region of space having a substantially constant force magnitude and direction, and wherein the utility space is adjacent to the air gap;

(b) a substantially transparent flow channel positioned at least partially within the region of space having a substantially constant force magnitude and direction and for allowing the introduction of at least one particle;

(c) a computer system for gathering and analyzing data associated with the at least one particle; and

(d) a camera located at least partially within the utility space.

11. The device of claim 10 further comprising a light source located at least partially within the utility space.

12. A device for determining one or more particle physical characteristics, the device comprising:

(a) a force field device for subjecting at least one particle to at least one force field, the force field device comprising;

(1) a base;

(2) first and second magnets coupled to the base for generating a magnetic flux;

(3) first and second pole pieces coupled to the first and second magnets;

the first and second pole pieces comprising a substantially planar shape having a magnetic flux concentrating portion; and

(4) the first and second pole pieces further configured to provide an air gap and a utility space for accommodating data collection equipment, wherein the air gap at least partially forms a region of space having a substantially constant force magnitude and direction, and wherein the utility space is adjacent to the air gap;

(b) a substantially transparent flow channel positioned at least partially within the region of space having a substantially constant force magnitude and direction and for allowing the introduction of at least one particle;

(c) a computer system for gathering and analyzing data associated with the at least one particle; and

(d) a light source located at least partially within the utility space.