Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces

• US 6,413,401 B1
• Filed: 07/29/1999
• Issued: 07/02/2002
• Est. Priority Date: 07/03/1996
• Status: Expired due to Term

• Current Assignee: Caliper Holdings Incorporated
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Parce, J. Wallace, Chow, Calvin Y. H.
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/214,127
• Time in Patent Office: 1,069 Days
• Field of Search: 204/450, 204/451, 204/452, 204/456, 204/466, 204/600, 204/601, 204/606, 204/616, 430/314, 216/16-18, 298/25, 295/921, 296/031.6, 296/031, 296/031.9, 296/037, 296/031.3
• US Class Current: 204/602
• CPC Class Codes: G01N 27/44713 Particularly adapted electric power supply
• CPC Class Codes: G01N 27/44752 Controlling the zeta potential, e.g. by wall coatings
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/02 Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

In a microfluidic system using electrokinetic forces, the present invention uses electrical current or electrical parameters, other than voltage, to control the movement of fluids through the channels of the system. Time-multiplexed power supplies also provide further control over fluid movement by varying the voltage on an electrode connected to a fluid reservoir of the microfluidic system, by varying the duty cycle during which the voltage is applied to the electrode, or by a combination of both. A time-multiplexed power supply can also be connected to more than one electrode for a savings in cost.

Claims

1. A microfluidic system comprising

a plurality of capillary channels in an insulated substrate;

a plurality of electrodes at different nodes of said capillary channels for creating electric fields in said capillary channels to electrokinetically flow materials in a fluid through said capillary channels;

at least one variable voltage controller operably coupled to said plurality of electrodes which is configured to control an applied voltage at said plurality of electrodes in response to a measure of electric current flow at said plurality of electrodes; and

at least one conductive lead on said substrate extending to a capillary channel location for determining a voltage level at said location due to said applied voltage.

2. The microfluidic system of claim 1 wherein said conductive lead is dimensioned such that a voltage across the lead in the capillary channel is less than about 1 volt due to said applied voltage.

3. The microfluidic system of claim 1 wherein said conductive lead is dimensioned such that a voltage across the lead in the capillary channel is less than about 0.1 volt due to said applied voltage.

4. The microfluidic system of claim 1 wherein said conductive lead is arranged to form a voltage divider circuit on said substrate so that voltage received from said conductive lead is a fraction of said voltage at said capillary channel location.

5. The microfluidic system of claim 1 further comprising an insulating plate covering said substrate, said conductive lead extending to an edge of said substrate.

6. The microfluidic system of claim 1 wherein said variable voltage controller comprises at least one time-multiplexed power supply.

7. The microfluidic system of claim 6 further comprising two or more time-multiplexed power supplies.

8. The microfluidic system of claim 7 wherein each of said two or more power supplies are operably coupled to three or more electrodes.

9. The microfluidic system of claim 1 wherein said conductive lead has a width of less than about 1 μ

m.

10. The microfluidic system of claim 1 wherein said conductive lead has a width sufficiently narrow along said capillary channel to avoid electrolysis due to said applied voltage.

11. A method of a manufacturing a microfluidic system comprising

disposing a plurality of capillary channels in an insulating substrate;

selecting a plurality of different nodes of said capillary channel to receive electrodes, said electrodes being capable of creating electric fields in said capillary channels in response to an applied voltage to said electrodes to electrokinetically flow materials in a fluid through said capillary channels;

fixing a plurality of electrodes at said different nodes of said capillary channels;

connecting said electrodes to at least one variable voltage controller which is configured to control the applied voltage at said electrodes in response to a measure of electric current flow at said electrodes; and

disposing at least one conductive lead on said substrate to a capillary channel location to determine a voltage at said capillary channel location due to said applied voltage at said plurality of electrodes.

12. The method of claim 11 wherein in said conductive lead disposing step, said conductive lead is dimensioned such that a voltage across the lead in the capillary channel is less than about 1 volt due to said applied voltage.

13. The methods of claim 12 wherein in said conductive lead disposing step, said conductive lead is dimensioned such that a voltage across the lead in the capillary channel is less than about 0.1 volt due to said applied voltage.

14. The method of claim 11 wherein said conductive lead disposing step further comprises arranging said conductive lead to form a voltage divider circuit on said substrate so that voltage received from said conductive lead is a fraction of said voltage at said capillary channel location.

15. The method of claim 11 wherein said conductive lead disposing step further comprises extending said conductive lead to an edge of said substrate, and further comprising covering said substrate with an insulating plate.