Biosensor

• US 6,416,641 B1
• Filed: 06/09/1999
• Issued: 07/09/2002
• Est. Priority Date: 06/11/1998
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro, Watanabe, Motokazu
• Primary Examiner(s): Tung, T.
• Application Number:
  US09/328,511
• Time in Patent Office: 1,126 Days
• Field of Search: 204/403, 204/416, 205/777.5
• US Class Current: 204/403.04
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

The present invention relates to a biosensor comprising a working electrode base plate 11, a counter electrode base plate 14 and a reagent layer containing at least an enzyme and an electron mediator, wherein a working electrode 12 disposed on the working electrode base plate and a counter electrode 15 disposed on the counter electrode base plate are positioned so as to mutually face and a terminal of a measuring device can be brought in contact with terminals 13, 16 of both electrodes from through-holes 25, 24. Such a biosensor can produce highly reliable and accurate measuring results with only a small amount of a sample.

Claims

1. A biosensor comprising:

a working electrode base plate,

a counter electrode base plate having a curved section,

a reagent layer containing at least an enzyme and an electron mediator,

a working electrode disposed on said working electrode base plate, and

a counter electrode disposed on said counter electrode base plate,

said working electrode and said counter electrode being positioned so as to mutually face one another with a space between the electrodes, said space being formed by the curved section of said counter electrode base plate.

2. A biosensor comprising:

a working electrode base plate,

a counter electrode base plate having a bottom surface, an upper surface and a side surface, said counter electrode base plate having a cut-away portion which provides access to an electrode terminal on the working electrode base plate through the counter electrode base plate,

a spacer member disposed between said working electrode base plate and said counter electrode base plate, and

a reagent layer containing at least an enzyme and an electron mediator,

a working electrode disposed on said working electrode base plate, and

a counter electrode disposed on said counter electrode base plate, said counter electrode being disposed on said bottom surface of said counter electrode base plate,

said working electrode and said counter electrode being disposed on opposite sides of said spacer member,

wherein at least a portion of at least one of said side surface and said upper surface of said counter electrode base plate being operative as an electrode terminal of the counter electrode.

3. The biosensor in accordance with claim 2, wherein said counter electrode is electrically connected to said upper surface via said side surface.

4. The biosensor in accordance with claim 2, wherein said counter electrode base plate has a through-hole filled with a conductive material for electrically connecting said counter electrode to said upper surface.

5. A biosensor comprising:

an insulating plate provided with a groove on its surface, said groove having a first side wall and a second side wall, said first side wall and said second side wall opposing one another,

a cover member coupled to said insulating base plate, said insulating plate and said cover member forming a space for accommodating a sample in said groove,

a working electrode formed in said insulating plate, and

a counter electrode formed in said insulating plate, said working electrode being disposed in said first side wall of said groove and said counter electrode being disposed in said second side wall of said groove, such that said working electrode and said counter electrode face one another,

and a reagent layer containing at least an enzyme and an electron mediator disposed in said groove.

6. The biosensor of claim 5, wherein said cover member further comprises an air vent disposed in a position corresponding to an inner most portion of said groove.

7. A biosensor comprising:

a working electrode base plate,

a counter electrode base plate having an upper surface, a bottom surface and a side surface,

a spacer member disposed between said working electrode base plate and said counter electrode base plate, and

a reagent layer containing at least an enzyme and an electron mediator,

a working electrode disposed on said working electrode base plate, and

a counter electrode disposed on said counter electrode base plate, said counter electrode being disposed on said bottom surface of said counter electrode base plate,

said working electrode and said counter electrode are disposed on opposite sides of said spacer member,

said counter electrode base plate having a through-hole formed therein which is filled with a conductive material, said conductive material contacting said counter electrode, and

said counter electrode base plate having a cut-away portion which provides access to an electrode terminal on the working base plate through the counter electrode base plate.

8. The biosensor of claim 7, wherein a first lead is coupled to said electrode terminal on the working base plate, and a second lead is coupled to the conductive material, both said first lead and said second lead being exposed to the upper surface of said counter electrode base plate.