Beta-1,3-galactosyltransferase homologs

• US 6,416,988 B1
• Filed: 12/10/1999
• Issued: 07/09/2002
• Est. Priority Date: 12/10/1998
• Status: Expired due to Fees

• Current Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company)
• Inventors: Jaspers, Stephen R., Gao, Zeren, Conklin, Darrell C., Yamamoto, Gayle
• Primary Examiner(s): Prouty, Rebecca E.
• Assistant Examiner(s): Steadman, David J.
• Application Number:
  US09/459,133
• Time in Patent Office: 942 Days
• Field of Search: 435/320.1, 435/325, 435/252.3, 435/193, 536/23.2
• US Class Current: 435/193
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)

Abstract

The present invention relates to polynucleotide and polypeptide molecules for znssp2, a novel member of the galactosyltransferase family. The polypeptides, and polynucleotides encoding them, are cell-cell interaction and glycoprotein synthesis modulating and may be used for delivery and therapeutics. The present invention also includes antibodies to the znssp2 polypeptides.

Claims

1. An isolated polynucleotide encoding a polypeptide wherein the polypeptide comprises residues 19 to 397 SEQ ID NO:

2.

2. The isolated polynucleotide according to claim 1, wherein the polypeptide molecule comprises residues 1 to 397 of SEQ ID NO:

2.

4. An expression vector comprising the following operably linked elements:

a transcription promoter;

a DNA segment wherein the DNA segment is the polynucleotide according to claim 1, and

a transcription terminator.

5. The expression vector according to claim 4 wherein the DNA segment additionally encodes an affinity tag.

6. A cultured cell transformed with an expression vector according to claim 4, wherein said cell expresses the polypeptide encoded by the DNA segment.

7. A method of producing a polypeptide comprising culturing a cell according to claim 6, whereby said cell expresses the polypeptide encoded by the DNA segment;

and recovering the polypeptide.

3. An isolated polynucleotide encoding a polypeptide molecule wherein the polypeptide is selected from the group consisting of;

a) a polypeptide consisting of residues 1 to 18 of SEQ ID NO;

2;

b) a polypeptide consisting of residues 19 to 147 of SEQ ID NO;

2;

c) a polypeptide comprising residues 19 to 397 of SEQ ID NO;

2; and

d) a polypeptide comprising residues 1 to 397 of SEQ ID NO;

2.