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Histological reconstruction and automated image analysis

DC
  • US 6,418,236 B1
  • Filed: 06/24/1999
  • Issued: 07/09/2002
  • Est. Priority Date: 06/24/1999
  • Status: Expired due to Term
First Claim
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1. A method for automated image analysis of a biological specimen, comprising:

  • (a) providing a biological sample to be analyzed;

    (b) automatically scanning the sample at a plurality of different positions with different coordinates;

    (c) automatically obtaining an image at each of said different coordinates to produce different images from different locations of the sample;

    (d) automatically detecting one or more targets of interest on the sample from the different images of the sample by automatically measuring one or more parameters of the sample and automatically comparing the measured parameters against selected threshold values of the parameters for the targets of interest;

    (e) automatically reconstructing an image of a selected target on the sample from the obtained different images, wherein a reconstructed image of a selected target spreading in two or more spatially adjacent and different images of the sample is reconstructed by using image information from the spatially adjacent and different images; and

    (f) processing the reconstructed image to obtain information of the selected target of interest.

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