Degrading sludge with microorganisms ATCC 55926 OR 202050

• US 6,420,165 B1
• Filed: 05/24/2000
• Issued: 07/16/2002
• Est. Priority Date: 11/26/1997
• Status: Expired due to Fees

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Weinstein, Bernard I., Figurski, David, Nakanishi, Koji, Hoshina, Sadayori
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Ware, Deborah K.
• Application Number:
  US09/577,640
• Time in Patent Office: 783 Days
• Field of Search: 435/243, 435/252.1, 435/822, 435/262.5, 435/261, 435/264, 435/832
• US Class Current: 435/262.5
• CPC Class Codes: C02F 3/34 characterised by the microorganisms used
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/07 Bacillus
• CPC Class Codes: Y10S 435/832 Bacillus

Abstract

A biologically pure culture of a microorganism is provided designated SH2A and deposited under ATCC Accession No. 55926, or a mutant derived therefrom. Further provided is a biologically pure culture of a microorganism designated SH2B and deposited under ATCC Accession No. 202050, or a mutant derived therefrom. A method of degrading an organic material such as sludge is carried out by treating the organic material with an effective, degrading amount of either SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom. The microorganism designated SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom, is grown by culturing the microorganism at a temperature and in a medium effective to promote growth of the microorganism.

Claims

1. A method of degrading an organic material contained in a sludge which comprises treating the sludge with an effective, degrading amount of a microorganism selected from the group consisting of a microorganism strain SH2A ATCC Accession No. 55926 microorganism strain SH2B ATCC Accession No. 202050 and mutants derived therefrom which retain the degrading activity thereof, so as to thereby degrade the material in the sludge.

2. The method of claim 1, wherein the organic material is a plastic material.

3. The method of claim 2, wherein the plastic material is polyethylene.

4. The method of claim 3, wherein the polyethylene is irradiated prior to treatment with the microorganism.

5. The method of claim 4, wherein the irradiation comprises exposing the polyethylene to ultra-violet light.

6. The method of claim 1, wherein the organic material is a Dioxin or a polychlorobiphenyl.

7. The method of claim 6, wherein the Dioxin is dibenzofuran.

8. The method of claim 1, wherein the treatment is effected at a temperature from about 62°

C. to about 100°

C.

9. The method of claim 8, wherein the temperature is about 62°

C.

10. The method of claim 1, wherein the treatment is effected at a pH at about 5.0 to about 8.0.

11. The method of claim 10, wherein the pH is about 7.4.

12. The method of claim 1, wherein the treatment is effected in an aerobic environment.