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Normalization control for hybridization reactions

  • US 6,423,535 B1
  • Filed: 11/24/1999
  • Issued: 07/23/2002
  • Est. Priority Date: 11/24/1999
  • Status: Expired due to Fees
First Claim
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1. A hybridization assay using an array comprising samples separately arrayed on and stably associated with a surface of a solid support, each sample comprising a distinct sample polynucleotide target and a standard polynucleotide target, wherein the sample target and the standard target arm co-arrayed to the same location on the array, the method comprising the steps of:

  • contacting each sample of the anay with a detectable sample probe specific for the sample target, and an independently detectable standard probe specific for the standard target, under hybridization conditions to produce a hybridization pattern comprising a sample probe signal and a standard probe signal at each sample, wherein the standard probe signal provides a normalization control for the sample signal; and

    detecting the hybridization pattern.

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