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Multichromophore fluorescent probes using DNA intercalation complexes

  • US 6,428,667 B1
  • Filed: 10/10/2000
  • Issued: 08/06/2002
  • Est. Priority Date: 03/14/1990
  • Status: Expired due to Fees
First Claim
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1. In a method for performing a separation of a composition comprising employing an electrical field for directing movement of components in said composition in a differential manner, the improvement which comprises:

  • having present during said separation in said electrical field a moiety comprising (a) a probe having a double stranded DNA of at least about 20 base pairs and (b) non-covalently bound intercalated in said double stranded DNA at least one fluorescent molecule characterized by having at least two positive charges, having at least one fluorescent monomeric unit and having a binding affinity to double stranded DNA of at least about 5×

    106 M

    1
    and up to about 1 fluorescent unit per 4 nucleotides in a nucleic acid strand, wherein said fluorescent unit is characterized by having at least one aromatic ring per monomeric unit.

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