Method for determination of hemoglobins
First Claim
1. A method for determining hemoglobins by a cation exchange LC characterized by the use of an eluent containing at least one chaotropic ion selected from the group consisting of tribromo acetate, trichloro acetate, thiocyanate, iodide, perchlorate, dichloroacetate, nitrate, bromide, barium, cesium and guanidine ions, and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.
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Abstract
A method for determining hemoglobins is provided which is suitable for determination of stable hemoglobin A1c. A method for determining hemoglobins by cation exchange liquid chromatography wherein an eluent is used containing a chaotropic ion and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.
39 Citations
11 Claims
- 1. A method for determining hemoglobins by a cation exchange LC characterized by the use of an eluent containing at least one chaotropic ion selected from the group consisting of tribromo acetate, trichloro acetate, thiocyanate, iodide, perchlorate, dichloroacetate, nitrate, bromide, barium, cesium and guanidine ions, and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.
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2. A method for determining hemoglobins by a cation exchange LC characterized by the use of an eluent containing at least one chaotropic ion selected from the group consisting of tribromo acetate, trichloro acetate, thiocyanate, iodide, perchlorate, dichloroacetate, nitrate, bromide, barium, cesium and guanidine ions, and a buffer agent having acid dissociation constants (pKa) in the range of 2.15-6.39 and in the range of 6.40-10.50.
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10. A method for determining hemoglobins by a cation exchange LC comprising the use of an eluent containing a chaotropic ion, at least one type of amine having a molecular weight of 20-500, and further an inorganic acid, an inorganic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.
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11. A method for determining hemoglobins by a cation exchange LC comprising the use of an eluent containing a chaotropic ion, a buffer agent having acid dissociation constants (pKa) in the range of 2.15-6.39 and in the range of 6.40-10.50, and at least one type of amine having a molecular weight of 20-500.
Specification