Apparatus and method of monitoring an analyte

• US 6,428,748 B1
• Filed: 01/31/2001
• Issued: 08/06/2002
• Est. Priority Date: 01/31/2001
• Status: Expired due to Fees

• Current Assignee: Grouptek Incorporated
• Original Assignee: Grouptek Incorporated
• Inventors: Wallach, Donald F. H.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Siefke, Samuel P
• Application Number:
  US09/774,242
• Time in Patent Office: 552 Days
• Field of Search: 422/56, 422/55, 422/57, 422/61, 422/68.1, 422/82.05, 422/82.08, 436/2, 436/164, 436/166, 436/169, 436/172
• US Class Current: 422/421
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

A detector for monitoring an analyte includes an analyte-sensing composition. The analyte-sensing composition has a visible color intensity or emission intensity (e.g., fluorescence intensity) that changes as the analyte concentration contacting the detector changes. The intensity changes can be visible to the human eye, or identified by an instrument. The analyte can include carbon dioxide, a volatile amine or a volatile carboxylic acid.

Claims

1. A detector for monitoring a sample for the presence of an analyte comprising:

a carrier having a thickness;

an analyte-sensing composition including an indicator dye, a buffer, and an osmotic control agent, the analyte-sensing composition being contained by the carrier and the buffer and indicator dye are present in a molar ratio of between 20 and 200;

a hydrophobic barrier on one surface of the carrier, the hydrophobic barrier being arranged to contact a test material, and

an outer barrier covering a surface of the carrier opposite the hydrophobic barrier.

2. The detector of claim 1, wherein the indicator dye includes an emissive dye.

3. The detector of claim 2, wherein the emissive dye includes fluorescein complexon.

4. The detector of claim 1, wherein the indicator dye includes a colored dye.

5. The detector of claim 4, wherein the colored dye includes a phthalic acid derivative.

6. The detector of claim 5, wherein the phthalic acid derivative includes o-cresolphthalein, phenolphthalein, or thymolphthalein.

7. The detector of claim 1, wherein the outer barrier is impermeable to water and gases and is transparent to visible light.

8. The detector of claim 1, wherein the hydrophobic barrier includes a hydrophobic silane coating, a fluorocarbon coating, or a laminated hydrophobic sheet.

9. The detector of claim 1, wherein the carrier is porous or fibrous.

10. The detector of claim 1, wherein the carrier has a thickness of less than 0.5 mnm.

11. The detector of claim 1, wherein the carrier has a thickness of less than 0.3 mm.

12. The detector of claim 1, further comprising a permeation modulator covering a portion of the hydrophobic barrier.

13. The detector of claim 12, wherein the permeation modulator has a lower permeability than hydrophobic layer or the carrier.

14. The detector of claim 13, wherein the permeation modulator has a thickness between 0.001 and 5 mm.

15. The detector of claim 13, wherein the permeation modulator has a thickness between 0.01 and 1 mm.

16. The detector of claim 1, wherein the osmotic control agent includes an alcohol.

17. The detector of claim 1, wherein the buffer includes an amino acid.

18. The detector of claim 1, wherein the buffer and indicator dye are present in a molar ratio of between 40 and 100.

19. The detector of claim 1, wherein the analyte is carbon dioxide, a volatile carboxylic acid or a volatile amine.

20. The detector of claim 1, wherein the detector is a portion of a food package or a hygiene product.

21. A detector for monitoring a sample for the presence of an analyte comprising:

a carrier of less than 0.5 mm;

an analyte-sensing composition including an indicator dye selected from the group consisting of an emissive dye or a colored dye, a buffer, and an osmotic control agent including an alcohol, the analyte-sensing composition being contained by the carrier and the buffer and indicator dye are present in a molar ratio of between 20 and 200;

a hydrophobic barrier on one surface of the carrier, the hydrophobic barrier being arranged to contact a test material, the hydrophobic barrier including a hydrophobic silane coating, a fluorocarbon coating, or a laminated hydrophobic sheet, and

an outer barrier covering a surface of the carrier opposite the hydrophobic barrier, the outer barrier being impermeable to water and gases and is transparent to visible light,

wherein the analyte is carbon dioxide, a volatile carboxylic acid or a volatile amine.

22. The detector of claim 21, further comprising a permeation modulator covering a portion of the hydrophobic barrier.

23. The detector of claim 22, wherein the permeation modulator has a lower permeability than hydrophobic layer or the carrier.

24. The detector of claim 22, wherein the permeation modulator has a thickness between 0.001 and 5 mm.

25. The detector of claim 23, wherein the permeation modulator has a thickness between 0.0 and 1 mm.

26. The detector of claim 21, wherein the buffer and indicator dye are present in a molar ratio of between 40 and 100.

27. The detector of claim 21, wherein the detector is a portion of a food package or a hygiene product.