Preparing artificial organs by forming polylayers of different cell populations on a substrate
First Claim
1. An artificial organ construct comprising:
- a biocompatable substrate;
a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatible substrate; and
a second cultured polylayer of cells derived from a second cell population that is different from the smooth muscle cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural biological structure upon implantation.
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Accused Products
Abstract
Compositions and methods are provided for producing multilayered artificial organs comprising heterogenous polylayers. Polylayers comprising homogenous cell populations are created on one side of a biocompatible substrate such that a chimeric interface is produced between the heterogenous polylayers. Cellular interaction at the chimeric interface produce an interstitial biomaterial with morphological and functional characteristics that resemble the natural in vivo organ. An artificial organ is produced by creating a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on a substrate in the shape of an organ, and creating a second cultured polylayer of cells derived from a cell population different from the smooth muscle cell population.
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Citations
31 Claims
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1. An artificial organ construct comprising:
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a biocompatable substrate;
a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatible substrate; and
a second cultured polylayer of cells derived from a second cell population that is different from the smooth muscle cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural biological structure upon implantation. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An artificial bladder construct comprising:
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a biocompatable substrate;
a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatable substrate; and
a second cultured polylayer of cells derived from a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural bladder upon implantation. - View Dependent Claims (9)
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10. A method for producing an artificial organ construct comprising:
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providing a biocompatable substrate in the shape of an organ;
creating a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatable substrate, wherein the first polylayer is attached to the biocompatable substrate; and
creating a second cultured polylayer of cells derived from a second cell population that is different from the smooth muscle cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural biological structure upon implantation, thereby producing an artificial organ construct. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for producing an artificial bladder construct comprising:
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providing a biocompatable substrate in the shape of a bladder;
creating a first cultured polylayer derived from an isolated population of smooth muscle cells on the biocompatable substrate, wherein the first polylayer is attached to the biocompatable substrate; and
creating a second cultured polylayer comprising a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer, such that the construct provides the functional equivalent of a natural bladder upon implantation, thereby producing an artificial bladder construct. - View Dependent Claims (22, 23, 24, 25)
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26. A method for treating a subject with a genitourinary disorder comprising:
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providing an artificial organ construct having a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on a biocompatable substrate, and second cultured polylayer of a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer;
implanting the organ construct into the subject, such that the construct provides the functional equivalent of a natural structure; and
monitoring the subject for a modulation in the genitourinary disorder. - View Dependent Claims (27, 28, 29, 30, 31)
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Specification