Process for the preparation of an herbal-therapeutic product extracted from the pulp of a species eugenia jambolana
First Claim
1. A process for the preparation of an herbal therapeutic product for controlling blood glucose levels, extracted from pulp of fruit of Eugenia jambolana, comprising cleaning and drying the fruit to remove extraneous material from the outer layer of the fruit, deseeding the fruit to form a de-seeded pulp, soaking the de-seeded pulp in water, mixing the water and pulp to form a mixture and keeping it overnight in controlled cooled conditions to maintain activity of hypoglycemic compounds present in the fruit pulp, filtering the mixture to form a residue, washing the residue with water to extract all active compounds in the form of a clear watery solution, reducing water content of the solution to obtain a filtrate in a concentrated form, purifying the filtrate by column chromatography using slurries of different column materials to elute the mixture of hypoglycemic compounds by adding different buffers to form a second mixture, conducting thin layer chromatography of the second mixture to separate the hypoglycemic compounds in the form of bands, exposing the bands in an iodine chamber for clear demarcation of the hypoglycemic compounds and extracting the hypoglycemic compounds to recover the herbal therapeutic product containing said hypoglycemic compounds for controlling blood glucose levels.
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Abstract
The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of fruit Eugenia jambolana. The process for the preparation of the herbal therapeutic product to control the glucose level, comprises cleaning and drying the fruit of a species of family Eugenia to remove extraneous material from the outermost layer of the said fruit. De-seeding the fruit and soaking the said de-seeded fruit in water under controlled cooled conditions overnight to retain the activity of hypoglycemic compounds in the mixture of water and pulp of de-seeded fruit. It is followed by mixing the mixture to achieve a uniform consistency.
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Citations
12 Claims
- 1. A process for the preparation of an herbal therapeutic product for controlling blood glucose levels, extracted from pulp of fruit of Eugenia jambolana, comprising cleaning and drying the fruit to remove extraneous material from the outer layer of the fruit, deseeding the fruit to form a de-seeded pulp, soaking the de-seeded pulp in water, mixing the water and pulp to form a mixture and keeping it overnight in controlled cooled conditions to maintain activity of hypoglycemic compounds present in the fruit pulp, filtering the mixture to form a residue, washing the residue with water to extract all active compounds in the form of a clear watery solution, reducing water content of the solution to obtain a filtrate in a concentrated form, purifying the filtrate by column chromatography using slurries of different column materials to elute the mixture of hypoglycemic compounds by adding different buffers to form a second mixture, conducting thin layer chromatography of the second mixture to separate the hypoglycemic compounds in the form of bands, exposing the bands in an iodine chamber for clear demarcation of the hypoglycemic compounds and extracting the hypoglycemic compounds to recover the herbal therapeutic product containing said hypoglycemic compounds for controlling blood glucose levels.
Specification
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- Resources
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Current AssigneeIndian Council of Medical Research
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Original AssigneeIndian Council of Medical Research
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InventorsSharma, Suman Bala, Nasir, Afreena, Prabhu, Krishan Madhav, Murthy, Pothapragada Suryanarayana
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Primary Examiner(s)TATE, CHRISTOPHER ROBIN
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Application NumberUS09/776,650Publication NumberTime in Patent Office547 DaysField of Search424/725, 424/777, 514/866US Class Current424/777CPC Class CodesA61K 36/61 Myrtaceae (Myrtle family), ...A61P 5/50 for increasing or potentiat...
