Method for alteration detection
First Claim
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1. A method for detecting an alteration in a target nucleic acid suspected to be in a biological sample, the method comprising the steps of:
- a) adding, to a biological sample suspected to contain a target nucleic acid, a plurality of single-stranded nucleic acids that hybridize contiguously to a region of said target nucleic acid if said region is unaltered;
b) adding to said biological sample an agent that degrades single-stranded nucleic acids; and
, c) detecting an alteration in said target nucleic acid as the presence of a degradation product from steps a) and b) resulting from degradation within said region of said target nucleic acid.
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Abstract
Methods are provided for detecting an alteration in a target nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease.
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Citations
22 Claims
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1. A method for detecting an alteration in a target nucleic acid suspected to be in a biological sample, the method comprising the steps of:
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a) adding, to a biological sample suspected to contain a target nucleic acid, a plurality of single-stranded nucleic acids that hybridize contiguously to a region of said target nucleic acid if said region is unaltered;
b) adding to said biological sample an agent that degrades single-stranded nucleic acids; and
,c) detecting an alteration in said target nucleic acid as the presence of a degradation product from steps a) and b) resulting from degradation within said region of said target nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification