SPR sensor cell and immunoassay apparatus using the same

• US 6,432,364 B1
• Filed: 07/06/1999
• Issued: 08/13/2002
• Est. Priority Date: 07/06/1998
• Status: Expired due to Fees

• Current Assignee: Suzuki Motor Corporation
• Original Assignee: Suzuki Motor Corporation
• Inventors: Suzuki, Kazuyasu, Negami, Mitsuhiro, Nakamura, Muneaki
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): SINES, BRIAN J
• Application Number:
  US09/348,613
• Time in Patent Office: 1,134 Days
• Field of Search: 422/82.11, 422/55-58, 422/82.08, 422/82.09, 422/82.06, 422/82.05, 385/12, 385/31, 385/39, 436/518
• US Class Current: 422/82.11
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658

Abstract

An SPR (surface plasmon resonance) sensor cell comprising: a light-transparent core; a clad covering the core and having a through hole at a predetermined position to communicate with the core; and a predetermined thin metal film formed on an exposed surface of the core corresponding to the through hole.

Claims

1. An SPR sensor cell comprising an optical waveguide, said optical waveguide comprising:

a plate-shaped first clad serving as a lower support member;

a core provided on a top surface of said first clad; and

a second clad to cover said core,

wherein a through hole is formed at a predetermined position of said second clad to communicate with a predetermined portion of said core, and wherein a predetermined thin metal film is formed on an exposed predetermined portion of said core surface corresponding to said through hole.

2. An SPR sensor cell of claim 1, wherein two or more said cores are provided and said second clad has a corresponding two or more said through holes.

3. An SPR sensor cell of claim 1, wherein said core has a longitudinal axis and an exposed face at each end of said longitudinal axis and on one of said two faces a light reflection surface is formed.

4. An SPR sensor cell of claim 1, wherein said core has a longitudinal axis and an exposed face at each end of said longitudinal axis and at least one of said end face surfaces of said core is inclined with respect to said longitudinal axis.

5. An SPR sensor cell of claim 1, wherein said second clad has a predetermined thickness such that said through hole functions as a container for a material being tested.

6. An SPR sensor cell of claim 1, wherein said core has a longitudinal axis and an exposed face at each end of said longitudinal axis and said two exposed faces have different areas.

7. An SPR sensor cell comprising:

at least two cores, each said core having a surface area serving as an SPR sensing portion;

a clad surrounding said cores except at a first end surface and a second end surface; and

at least one through hole formed in said clad so as to communicate simultaneously with at least two of said at least two SPR sensing portions.

8. An SPR sensor cell of claim 7, wherein said SPR sensor portion is formed at least at two locations of each said core and a plurality of said through holes are provided, wherein each said through hole is formed so as to simultaneously communicate with at least two said SPR sensing portions.

9. An SPR sensor of claim 7, wherein said clad has at least one end surface having a characteristic that reduces passage of light.

10. An immunoassay apparatus comprising:

an SPR sensor cell comprising;

at least two cores, each said core having a surface area serving as an SPR sensing portion;

a clad surrounding said cores except on two end surfaces; and

a through hole formed in said clad so as to communicate simultaneously with at least two of said SPR sensing portions;

a light source for emitting light into said cores; and

a spectrometer or photodiode for performing a wavelength distribution of the light emitted from said SPR sensor cell.

11. An immunoassay apparatus of claim 10, wherein said SPR sensing portion is formed on at least two locations of each of said cores and a plurality of said through holes are arranged so that each said through hole communicates simultaneously with adjacent SPR sensing portions on two adjacent cores.

12. An immunoassay apparatus of claim 10, wherein said light source emits light simultaneously into at least two of said at least two cores.

13. An immunoassay apparatus of claim 10, wherein said light source emits light successively into each said at least two cores.

14. An immunoassay apparatus of claim 10, wherein said light source is a white LED lamp or halogen lamp capable of emitting light of different wavelengths.

15. An immunoassay apparatus of claim 10, wherein said clad material has at least one outside surface having a characteristic that light is inhibited from passing through said outside surface.

16. An immunoassay apparatus comprising:

an SPR sensor cell having an SPR sensing portion and a through hole adjacent to said said sensing portion, wherein said through hole has a predetermined thickness dimension sufficient to permit said through hole to serve as a test material holding receptacle;

a light source for emitting light of a predetermined wavelength band into said SPR sensor cell; and

a light analyzing means for analyzing light which has passed through said SPR sensor cell,

wherein said light source comprises a white LED lamp and said SPR sensor cell comprises at least two cores and clads surrounding said cores on all sides except two end surfaces, said immunoassay apparatus further comprising;

an optical fiber introducing light which has passed through said cores into said light analyzing means; and

an optical coupler passing light from one of said cores to said optical fiber.

17. An immunoassay apparatus of claim 16, further comprising:

a plurality of light sources, respectively corresponding to said at least two cores, wherein said plurality of light sources are successively turned on one after another so as to introduce light successively to said corresponding respective core.

18. An immunoassay apparatus comprising:

an SPR sensor cell having an SPR sensing portion and a through hole adjacent to said said sensing portion, wherein said through hole has a predetermined thickness dimension sufficient to permit said through hole to serve as a test material holding receptacle;

a light source for emitting light of a predetermined wavelength band into said SPR sensor cell; and

a light analyzing means for analyzing light which has passed through said SPR sensor cell,

wherein said light source comprises a white LED lamp and said SPR sensor cell includes at least two cores arranged in a parallel manner on a plane, each said core surrounded by clads on all sides except two end faces, said light source is arranged in a vicinity of one of said two end faces of one of said SPR sensor cell cores, said light analyzing means arranged to receive light passing through the other of said two end faces, further comprising;

a mover to successively align each said core to said light source and said light analyzing means.

19. An immunoassay apparatus comprising:

an SPR sensor cell having an SPR sensing portion and a through hole adjacent to said said sensing portion, wherein said through hole has a predetermined thickness dimension sufficient to permit said through hole to serve as a test material holding receptacle;

a light source for emitting light of a predetermined wavelength band into said SPR sensor cell; and

a light analyzing means for analyzing light which has passed through said SPR sensor cell,

wherein said light source comprises a white LED lamp and an end surface of said SPR sensor cell where said light is introduced possesses a surface characteristic that tends to suppress introduction of light except for an end surface of said core.

20. An SPR sensor cell comprising:

a core having a rectangular cross section for passing light from a predetermined light source, said core thereby having four side surfaces and two end surfaces;

an SPR sensing portion on a predetermined location of one of said four side surfaces of said core; and

at least two clads formed so as to surround said four side surfaces of said core excluding the SPR sensing portion,

wherein two of said four side surfaces of said core have a low-reflection surface where a light reflection is lowered, said two side surfaces having said low-reflection surface are the two side surfaces other than said one side surface having said SPR sensing portion and a side surface opposite to said surface have said SPR sensing portion.

22. An SPR sensor cell of claim 20, wherein said low-reflection surface comprises an obscured glass.

23. An SPR sensor cell of claim 20, wherein said low-reflection surface comprises an aluminum coating.

24. An SPR sensor cell of claim 20, wherein said low-reflection surface comprises a black paint.

25. An SPR sensor cell of claim 20, wherein said four sides surrounded by said shroud are subjected to a surface treatment which makes it difficult to pass light.

26. An immunoassay apparatus comprising:

a SPR sensor cell disclosed in claim 20;

a light source for emitting a predetermined light into said core of said SPR sensor; and

a light analyzer for analyzing a wavelength distribution of the light which has passed through the core.

21. An SPR sensor cell comprising:

a core having a rectangular cross section for passing light from a predetermined light source, said core having an SPR sensing portion on one side surface thereof; and

at least two clads enshrouding said core excluding said SPR sensing portion, said at least two clads having a plurality of surfaces adjacent to said core,

wherein said surfaces of said clads facing the core have a low reflection ratio where a light reflection is lower than a light reflection in said core.

27. An SPR sensor cell comprising:

at least one sheet-shaped core transmitting light from a light source;

at least one SPR sensing portion on a top surface of said core;

a first clad upon which is mounted said core; and

a second clad having a through hole at a position corresponding to said SPR sensing portion, such that said core is sandwiched between said first clad and said second clad.

28. An SPR sensor of claim 27, wherein an end surface of the core possesses a surface characteristic that makes it difficult to pass the light from the light source (low light transmitting), excluding a core region corresponding to the SPR sensing portion.

29. An SPR sensor cell of claim 28, wherein a plurality of the SPR sensing portions are provided on the core.

30. An SPR sensor cell of claim 29, wherein said at least one core comprises a plurality of light-transparent members having a rectangular cross section and made from an identical material, and wherein light-transparent members having low light transmitting end surface and light-transparent members having no low light transmitting end surface are alternately arranged.

31. An SPR sensor cell of claim 30, wherein each of the light-transparent members having the low lights transmitting end surface has side surfaces (i.e., side surfaces adjacent to the light-transparent members having no low light transmitting end surface) which possess said surface characteristic that it is difficult to pass light.

32. An SPR sensor cell of claim 30, wherein each of the light-transparent members having no low light transmitting end surface has side surface (i.e., side surfaces adjacent to the light-transparent members having the low light transmitting end surface) which side surfaces possess said surface characteristic that it is difficult to pass the light.

33. An immunoassay apparatus comprising:

an SPR sensor cell comprising;

at least one sheet-shaped core transmitting light from a light source;

at least one SPR sensing portion on a top surface of said core;

a first clad upon which is mounted said core; and

a second clad having a through hole at a position corresponding to said SPR sensing portion, such that said core is sandwiched between said first clad and said second clad;

a light source for emitting light into the core of the SPR sensor cell; and

a light analyzing means for analyzing a wavelength distribution of the light emitted from the SPR sensor cell.

34. An immunoassay apparatus of claim 33, wherein at least one pin hole plate is provided in the vicinity of the SPR sensor cell in at least one of before the light enters the SPR sensor cell and after the light comes out of the SPR sensor cell.

35. An immunoassay apparatus of claim 33, further comprising:

a predetermined block member in the vicinity of the SPR sensor cell in at least one of before the light passes the SPR sensor and after the light passes the SPR sensor cell; and

an optical fiber for connecting the block member to the light source or to the light analyzing member, wherein an optical fiber fixing portion is formed in the block member corresponding to the core of the SPR sensor cell, and the optical fiber has an end portion covered with a ferrule, and fixed into the optical fiber fixing portion.