Analytical system and method

• US 6,432,720 B2
• Filed: 10/12/2001
• Issued: 08/13/2002
• Est. Priority Date: 08/02/1996
• Status: Expired due to Term

• Current Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Original Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Chow, Calvin Y. H.
• Primary Examiner(s): Drodge, Joseph W.
• Application Number:
  US09/977,032
• Publication Number:
  US 20020015667A1
• Time in Patent Office: 305 Days
• Field of Search: 422/58, 422/63, 422/68.1, 422/81, 422/82.05, 422/82.09, 422/99-104, 436/4-6, 436/7.21, 436/514, 435/2, 435/7.21, 435/7.2, 216/2, 216/56, 204/269, 204/403, 204/412, 204/450, 204/451, 204/600, 204/601, 210/97
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An analytical or preparatory system comprised as a base unit, an adapter, and a substrate. The adapter is attached to an attachment region on the base unit, and containing flow biasing connectors for delivering energy to selected regions of the substrate. The substrate is attached to an attachment region on the adapter. The adapter permits the base unit to be interfaced with a wide variety of different substrates to perform chemical and biological analytical analyses and preparatory procedures.

Claims

1. A microfluidic system, comprising:

a base unit;

an adapter plate removably and operably coupled to the base unit, the adapter plate comprising a plurality of flow biasing connectors operable for delivering energy in the form of pressure from the base unit to selected regions of a microfluidic substrate; and

the microfluidic substrate comprising a plurality of mesoscale channels disposed therein, and mounted against the adapter plate, whereupon the flow biasing connectors deliver pressure to selected regions of the microfluidic substrate to induce flow of fluids through the mesoscale channels of the microfluidic substrate.

2. The system of claim 1, wherein the microfluidic substrate is removably mounted against the adapter plate.

3. The system of claim 1, wherein the adapter plate is removably connected to the base unit.

4. The analytical system of claim 1, wherein the microfluidic substrate comprises a polymeric material.

5. The analytical system of claim 4, wherein the microfluidic substrate comprises polydimethylsiloxane.

6. The system of claim 1, further comprising a detector positioned adjacent to the microfluidic substrate to receive optical signals from at least one of the mesoscale channels.

7. The system of claim 6, wherein the detector is disposed within the base unit.

8. A microfluidic system, comprising:

a base unit comprising at least a first source of pressure energy;

an adapter plate comprising a plurality of flow biasing connectors, wherein the adapter plate is removably coupled to the base unit and the flow biasing connectors are operably coupled to the at least first source;

a microfluidic substrate comprising a plurality of mesoscale channels disposed therein, the microfluidic substrate being mounted against the adapter plate and positioned such that the flow biasing connectors communicate pressure energy to the microfluidic substrate to direct or manage flow in the plurality of mesoscale channels.

9. The system of claim 8, wherein the microfluidic substrate is removably mounted against the adapter plate.

10. The system of claim 8, wherein the adapter plate is removably connected to the first source of pressure energy.

11. The system of claim 8, wherein the microfluidic substrate comprises a polymeric material.

12. The system of claim 11, wherein the microfluidic substrate comprises polydimethylsiloxane (PDMS).

13. The system of claim 8, further comprising a detector positioned adjacent to the microfluidic substrate to receive optical signals from at least one of the mesoscale channels.

14. A method of controlling fluid flow in a microfluidic substrate that includes a plurality of mesoscale fluid channels disposed therein, comprising:

providing a base unit and an adapter plate removably coupled to the base unit, the adapter plate comprising a plurality of flow biasing connectors that are operably coupled to the base unit;

mounting the microfluidic substrate against the adapter plate; and

selectively delivering pressure energy from the base unit through the flow biasing connectors to regions of the microfluidic substrate to energize the regions of the substrate to induce or manage flow through the mesoscale fluid channels.

15. The method of claim 14, wherein the step of selectively delivering pressure energy comprises delivering pressure to different regions of the microfluidic substrate at different times to control fluid movement through the mesoscale channels.

16. The method of claim 14, wherein the step of selectively delivering pressure energy comprises delivering pressure energy to fluid in the mesoscale channels.

17. The method of claim 14, wherein the step of selectively delivering pressure energy comprises delivering energy to one or more reservoirs that are fluidly connected to the mesoscale channels.

18. The method of claim 14, wherein the microfluidic substrate comprises a polymeric material.

19. The method of claim 18, wherein the polymeric material comprises PDMS.

20. The method of claim 14, wherein the step of mounting the microfluidic substrate against the adapter plate comprises removably mounting the microfluidic substrate against the adapter plate.