Electrodes for the measurement of analytes in small sample volumes
First Claim
1. A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises:
- a non-conducting substrate;
a discontinuous conductive layer deposited on adjacent first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second portions;
an analyte-specific reagent coated on the conductive layer on the first portion;
a reference electrode on the the conductive layer on the second portion;
a spacer layer deposited over the conductive layer;
a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and
a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed portion of the mesh at one exterior edge of the mesh.
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Accused Products
Abstract
A device, for use in the electrochemical analysis of an analyte in a small volume liquid sample, having a non-conducting substrate (1); a conductive layer, deposited on the substrate, in two parts (2a, 2b), defining a non-conducting gap (8) therebetween; an analyte-specific reagent (5) coated on the conductive layer, on one side of the gap; a reference electrode (3) on the conductive layer, on the other side of the gap; a spacer layer (4) deposited over the conductive layer; a monofilament mesh (6) coated with a surfactant or chaotropic agent, the mesh being laid over the reagent, the reference electrode and the spacer layer; and a second non-conductive layer (7) adhered to the mesh layer, but not coextensive therewith, thereby providing a sample application area (9) on the mesh.
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Citations
11 Claims
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1. A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises:
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a non-conducting substrate;
a discontinuous conductive layer deposited on adjacent first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second portions;
an analyte-specific reagent coated on the conductive layer on the first portion;
a reference electrode on the the conductive layer on the second portion;
a spacer layer deposited over the conductive layer;
a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and
a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed portion of the mesh at one exterior edge of the mesh. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises:
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a non-conducting substrate;
a first and a second conductive layer deposited on first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second conductive layers;
an analyte-specific reagent coated on the first conductive layer;
a reference electrode on the second conductive layer;
a spacer layer deposited over a portion of both the first and second conductive layers;
a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and
a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed edge portion of the mesh at one exterior edge of the mesh.
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Specification