Parallel high-performance liquid chromatography with post-separation treatment
First Claim
1. A method for characterizing a plurality of polymer samples with a liquid chromatography system, the method comprisingsupplying a mobile phase in parallel through each of first and second chromatographic columns of a liquid chromatography system, injecting first and second polymer samples into the mobile phase of the first and second chromatographic columns, respectively, separating at least one sample component of the injected first and second samples from other sample components thereof in the respective chromatographic columns, treating the at least one separated sample component of the first and second samples to change a property of at least one separated sample component thereof, and detecting a property of the treated sample component of the first and second samples.
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Abstract
High-performance liquid chromatography (HPLC) methods and systems are disclosed that combine parallel chromatographic separation of a plurality of samples with a detection technique that involves post-separation treatment of the plurality of samples to enhance one or more properties of the sample or of a component thereof, followed by detection of the one or more enhanced properties. Selective, tunable detection schemes are achievable, and are particularly advantageous as applied in connection with combinatorial chemistry, combinatorial material science and more particularly, combinatorial synthesis and screening of polymeric materials.
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Citations
23 Claims
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1. A method for characterizing a plurality of polymer samples with a liquid chromatography system, the method comprising
supplying a mobile phase in parallel through each of first and second chromatographic columns of a liquid chromatography system, injecting first and second polymer samples into the mobile phase of the first and second chromatographic columns, respectively, separating at least one sample component of the injected first and second samples from other sample components thereof in the respective chromatographic columns, treating the at least one separated sample component of the first and second samples to change a property of at least one separated sample component thereof, and detecting a property of the treated sample component of the first and second samples.
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18. A method for characterizing a plurality of polymer samples with a liquid chromatography system, the method comprising
supplying a mobile phase in parallel through each of first and second chromatographic columns of a liquid chromatography system, injecting first and second polymer samples into the mobile phase of the first and second chromatographic columns, respectively, separating at least one sample component of the injected first and second polymer samples from other sample components thereof in the respective chromatographic columns, precipitating or derivatizing at least one separated sample component of the first and second polymer samples, and detecting an optical property of a precipitated or derivatized sample component of the first and second polymer samples.
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19. A method for characterizing a plurality of polymer samples with a liquid chromatography system, the method comprising
supplying a mobile phase in parallel through each of two or more chromatographic columns of a liquid chromatography system, the mobile phase comprising a solvent for ten or more polymer samples of interest, injecting the ten or more different polymer samples of interest into the mobile phase of the two or more chromatographic columns, separating at least one sample component of the injected ten or more polymer samples from other sample components thereof in the respective chromatographic columns, combining the eluant of the chromatographic column with a non-solvent for the ten or more polymer samples of interest to precipitate the at least one separated sample component thereof, and detecting an optical property of the precipitated sample component of the ten or more polymer samples.
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20. A method for characterizing a plurality of polymer samples with a liquid chromatography system, the method comprising
supplying a mobile phase in parallel through each of first and second chromatographic columns of a liquid chromatography system, injecting first and second polyer samples into the mobile phase of the first and second chromatographic columns, respectively, separating at least one sample component of the injected first and second samples from one or more other sample components thereof in the respective chromatographic columns, controlling the composition or the temperature of the mobile-phase after chromatographic separation, such that an optical property of one or more of the separated sample components of the first and second samples is selectively changed relative to the optical properties of other sample components of the first and second samples, and selectively detecting the changed optical property of the at least one separated sample component of the first and second samples.
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21. A method for characterizing a plurality of samples with a flow-injection analysis system, the method comprising
supplying a mobile phase in parallel through each of first and second flow detectors of a flow-injection analysis system, injecting first and second polymer samples into the mobile phase of the first and second flow detectors, respectively, controlling the composition, the flow-rate or the temperature of the mobile-phase after injection of the first and second polymer samples, such that an optical property of one or more of the sample components of the first and second polymer samples is selectively changed relative to the optical properties of other sample components of the first and second polymer samples, and selectively detecting the changed optical property of the at least one sample component of the first and second polymer samples.
Specification