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Device and method for integrated diagnostics with multiple independent flow paths

  • US 6,436,722 B1
  • Filed: 04/18/2000
  • Issued: 08/20/2002
  • Est. Priority Date: 04/18/2000
  • Status: Expired due to Fees
First Claim
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1. A device for performing an assay which determines a presence or quantity of an analyte in a liquid sample (1) using a solid phase for capturing said analyte or the analyte bound to a binding partner for the analyte, and (2) detecting binding of said analyte or said analyte bound to its binding partner after washing unbound material from said solid phase, said device comprising:

  • (a) a first flow path for sample entry and delivery to a solid phase zone (c);

    (b) a second flow pot comprising a fluid path material, said fluid path material essentially impermeable to said liquid sample, but which defines a second flow path for said sample when made permeable by contact with a wash reagent, wherein said second flow path is independent of said first flow path;

    (c) a solid phase zone permeable to said liquid sample, at an intersection between said first and second flow paths, wherein said solid phase zone comprises a binding partner for said analyte or said analyte bound to its binding partner, and said solid phase is used in both capture and detection of said analyte or said analyte bound to its binding partner, whereby said liquid sample initially flows only along said first flow path into said sold phase zone but not into said fluid path material until said fluid path material is rendered hydrophilic by introduction of said wash reagent into said second flow path, whereby said wash reagent removes unbound material from said solid phase zone through said second flow path; and

    (d) a means to test a single sample for a single analyte or a means to test a single sample for multiple analytes.

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