Preparation of microbial polyunsaturated fatty acid containing oil from pasteurized biomass

• US 6,441,208 B2
• Filed: 01/19/2001
• Issued: 08/27/2002
• Est. Priority Date: 03/28/1996
• Status: Expired due to Term

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM B.V.
• Inventors: Visser, Johannes Martinus Jacobus, Bijl, Hendrik Louis, Schaap, Albert, Wolf, Johannes Hendrik
• Primary Examiner(s): Carr, Deborah D
• Application Number:
  US09/764,087
• Publication Number:
  US 20010025114A1
• Time in Patent Office: 585 Days
• Field of Search: 554/8, 554/20, 435/132, 435/134, 435/171, 435/254.1, 435/257.1
• US Class Current: 554/8
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 33/11 Plant sterols or derivatives thereof, e.g. phytosterols
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23L 33/15 Vitamins
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/1868 Docosahexaenoic acid
• CPC Class Codes: A23V 2250/1882 Polyunsaturated fatty acids
• CPC Class Codes: A23V 2250/706 Vitamin B12
• CPC Class Codes: A61K 2800/85 Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
• CPC Class Codes: A61K 8/375 the alcohol moiety containing more than one hydroxy group
• CPC Class Codes: A61K 8/67 Vitamins
• CPC Class Codes: A61K 8/92 Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C07C 51/47 by solid-liquid treatment; by chemisorption
• CPC Class Codes: C07C 57/03 Monocarboxylic acids
• CPC Class Codes: C07C 57/12 Straight chain carboxylic acids containing eighteen carbon atoms
• CPC Class Codes: C11B 1/02 Pretreatment
• CPC Class Codes: C11B 1/10 : by extracting
• CPC Class Codes: C11B 1/104 : using super critical gases or vapours
• CPC Class Codes: C12N 1/005 : after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12P 17/06 : containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 19/42 : Cobalamins, i.e. vitamin B12, LLD factor
• CPC Class Codes: C12P 23/00 : Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00
• CPC Class Codes: C12P 7/6427 : Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12P 7/6432 : Eicosapentaenoic acids [EPA]
• CPC Class Codes: C12P 7/6434 : Docosahexenoic acids [DHA]
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6472 : containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurized fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

Claims

1. A process for the isolation of one or more compound(s) from a microbial biomass, the process comprising:

a) culturing microorganisms in a fermentation broth under conditions whereby the microorganisms produce the compound;

b) pasteurising either the fermentation broth or a microbial biomass derived therefrom; and

c) extracting, isolation or recovering the compound from the microbial biomass.

2. A process according to claim 1 wherein the pasteurisation at least partially inactivates one or more compound degrading substances either in the biomass or in the broth.

3. A process according to claim 2 wherein the substance is a protease or a triglyceride degrading enzyme.

4. A process according to claim 3 wherein the enzyme is a lipase, a phospholipase, or a lipoxygenase.

5. A process according to claim 1 wherein pasteurisation comprises heating at from 50 to 100°

C.

6. A process according to claim 5 wherein the heating is at from 65 to 95°

C.

7. A process according to claim 5 wherein the heating is for from 30 to 90 minutes.

8. A process according to claim 1 wherein the fermentation broth is pasteurised while still in the fermentation vessel.

9. A process according to claim 1 wherein pasteurisation takes place after fermentation has finished.

10. A process according to claim 1 wherein the compound comprises a triglyceride.

11. A process according to claim 1 wherein the compound comprises at least one polyunsaturated fatty acid (PUFA).

12. A process according to claim 8 wherein, after pasteurisation, the fermentation broth is dewatered and/or filtered to produce the microbial biomass.

13. A process according to claim 12 wherein the biomass is treated, if necessary, to give a dry matter content of from 25 to 80%.

14. A process according to claim 13 wherein the resulting biomass is granulated into granular particles, and the granular particles are then dried.

15. A process for the isolation of at least one polyunsaturated fatty acid (PUFA) from a microbial biomass, the process comprising:

a) culturing microorganisms in a fermentation broth under conditions whereby the microorganisms produce the PUFA;

b) pasteurising either the fermentation broth or a microbial biomass derived therefrom; and

c) extracting, isolating or recovering the PUFA from the microbial biomass.

16. A process according to claim 15 wherein the pasteurisation at least partially inactives one or more PUFA degrading substances present either in the biomass or in the broth.

17. A process according to claim 16 wherein the substance is a protease or a triglyceride degrading enzyme.

18. A process according to claim 17 wherein the enzyme is a lipase, a phospholipase, or a lipooxygenase.

19. A process according to claim 15 wherein pasteurisation comprises heating at from 50 to 100°

C.

20. A process according to claim 19 wherein the heating is at from 65 to 95°

C.

21. A process according to claim 19 wherein the heating is for from 30 to 90 minutes.

22. A process according to claim 15 wherein the fermentation broth is pasteurized while still in the fermentation vessel.

23. A process according to claim 15 wherein pasteurisation take place after fermentation has finished.

24. A process according to claim 15 wherein the PUFA is in the form of a triglyceride.

25. A process according to claim 15 wherein after pasteurisation, the fermentation broth is dewatered and/or filtered to produce the microbial biomass.

26. A process according to claim 25 wherein the biomass is treated, if necessary, to give a dry matter content of from 25 to 80%.

27. A process according to claim 26 wherein the resulting biomass is granulated into granular particles, and the granular particles are the dried.

30. A PUFA or a microbial oil which has been extracted or isolated by a process according to claim 15.

28. A composition comprising granular particles, the particles having been formed by granulating a microbial biomass containing at least one polyunsaturated fatty acid (PUFA).

29. A composition comprising dried granules formed by drying granular particles according to claim 28.

31. A PUFA or a microbial oil which has been prepared from the dried granules of claim 29.