Functionalization of substrate surfaces with silane mixtures
First Claim
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1. A process for synthesizing an oligomer array on a substrate surface, comprising:
- (a) functionalizing a substrate surface by contacting a substrate having reactive hydrophilic moieties on the surface thereof with a derivatizing composition comprising a first silane R1—
Si(RLRxRy) and a second silane R2—
(L)n—
Si(RLRxRy) under reaction conditions effective to couple the silanes to the substrate surface and provide —
Si—
R1 groups and —
Si—
(L)n—
R2 groups thereon, wherein the RL, moieties, which may be the same or different, are groups which leave the silanes during the coupling, the Rx and Ry are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that upon binding to the substrate surface lowers the surface energy thereof, n is 0 or 1, L is a linking group, and R2 is a functional group enabling covalent binding of a molecular moiety or a modifiable group that is capable of being converted to such a functional group; and
(b) synthesizing a plurality of substrate-bound oligomers at each R2 by sequentially coupling monomers thereto, wherein array features are created by the separation of individual coupling reactions as the oligomers are synthesized so as to form an oligomer array on the functionalized surface provided in (a);
wherein the substrate comprises a polymeric material, silica, glass, or ceramic.
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Abstract
Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.
73 Citations
13 Claims
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1. A process for synthesizing an oligomer array on a substrate surface, comprising:
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(a) functionalizing a substrate surface by contacting a substrate having reactive hydrophilic moieties on the surface thereof with a derivatizing composition comprising a first silane R1—
Si(RLRxRy) and a second silane R2—
(L)n—
Si(RLRxRy) under reaction conditions effective to couple the silanes to the substrate surface and provide —
Si—
R1 groups and —
Si—
(L)n—
R2 groups thereon, wherein the RL, moieties, which may be the same or different, are groups which leave the silanes during the coupling, the Rx and Ry are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that upon binding to the substrate surface lowers the surface energy thereof, n is 0 or 1, L is a linking group, and R2 is a functional group enabling covalent binding of a molecular moiety or a modifiable group that is capable of being converted to such a functional group; and
(b) synthesizing a plurality of substrate-bound oligomers at each R2 by sequentially coupling monomers thereto, wherein array features are created by the separation of individual coupling reactions as the oligomers are synthesized so as to form an oligomer array on the functionalized surface provided in (a);
wherein the substrate comprises a polymeric material, silica, glass, or ceramic. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 10)
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8. A process for preparing an oligomer array, comprising:
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(a) functionalizing a substrate surface by contacting a substrate having reactive hydrophilic moieties on the surface thereof with a derivatizing composition comprising a first silane R1—
Si(RLRxRy) and a second silane R2—
(L)n—
Si(RLRxRy) under reaction conditions effective to couple the silanes to the substrate surface and provide —
Si—
R1 groups and —
Si—
(L)n—
R2 groups thereon, wherein the RL, moieties, which may be the same or different, are groups which leave the silanes during the coupling, the Rx and Ry are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that upon binding to the substrate surface lowers the surface energy thereof, n is 0 or 1, L is a linking group, and R2 is a functional group enabling covalent binding of a molecular moiety or a modifiable group that is capable of being converted to such a functional group; and
(b) binding a plurality of oligomers to the functionalized substrate by coupling an oligomer to each R2;
wherein the substrate comprises a polymeric material, silica, glass, or ceramic. - View Dependent Claims (11)
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12. A process for synthesizing an oligomer array on a substrate surface, comprising:
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(a) functionalizing a substrate surface by contacting a substrate having reactive hydrophilic moieties on the surface thereof with a derivatizing composition comprising a first silane R1—
Si(RLRxRy) and a second silane R2—
(L)n—
Si(RLRxRy) under reaction conditions effective to couple the silanes to the substrate surface and provide —
Si—
R1 groups and —
Si—
(L)n—
R2 groups thereon, wherein the RL, moieties, which may be the same or different, are groups which leave the silanes during the coupling, the Rx and Ry are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that upon binding to the substrate space lowers the surface energy thereof, n is 0 or 1, L is a linking group, and R2 is a functional group enabling covalent binding of a molecular moiety or a modifiable group that is capable of being convened to such a functional group; and
(b) synthesizing a plurality of substrate-bound oligomers at each R2 by sequentially coupling monomers thereto, wherein array features are created by the separation of individual coupling reactions as the oligomers are synthesized so as to form an oligomer array on the functionalized surface provided in (a);
wherein the substrate has reactive hydrophilic moieities selected from any one or more of hydroxyl groups, carboxyl groups, thiol groups, and substituted or unsubstituted amino groups.
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13. A process for preparing an oligomer array, comprising:
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(a) functionalizing a substrate surface by contacting a substrate having reactive hydropbilic moieties on the surface thereof with a derivatizing composition comprising a first silane R1—
Si(RLRxRy) and a second silane R2—
(L)n—
Si(RLRxRy) under reaction conditions effective to couple the silanes to the substrate surface and provide —
Si—
R1 groups and —
Si—
(L)n—
R2 groups thereon, wherein the RL, moieties, which may be the same or different, are groups which leave the silanes during the coupling, the Rx and Ry are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that upon binding to the substrate surface lowers the surface energy thereof, n is 0 or 1, L is a linking group, and R2 is a functional group enabling covalent binding of a molecular moiety or a modifiable group that is capable of being converted to such a functional group; and
(b) binding a plurality of oligomers to the functionalized substrate by coupling an oligomer to each R2;
wherein the substrate has reactive hydrophilic moieities selected from any one or more of hydroxyl groups, carboxyl groups, thiol groups, and substituted or unsubstitited amino groups.
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Specification