Colorimeter and assay device

• US 6,445,451 B1
• Filed: 02/12/1999
• Issued: 09/03/2002
• Est. Priority Date: 02/12/1998
• Status: Expired due to Fees

• Current Assignee: Hamilton-Thorn Research
• Original Assignee: Hamilton-Thorn Research
• Inventors: Kenny, Thomas G., Douglas-Hamilton, Diarmaid H.
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): Punnoose, Roy M.
• Application Number:
  US09/249,943
• Time in Patent Office: 1,299 Days
• Field of Search: 356/319, 356/300, 356/402, 356/407, 356/408, 356/425, 356/244, 356/246
• US Class Current: 356/425
• CPC Class Codes: G01J 3/0243 having a through-hole enabling the optical element to fulfil an additional optical function, e.g. a mirror or grating having a throughhole for a light collecting or light injecting optical fiber
• CPC Class Codes: G01J 3/50 using electric radiation detectors
• CPC Class Codes: G01J 3/501 Colorimeters using spectrally-selective light sources, e.g. LEDs

Abstract

A cassette reader for detecting the status of stained samples is provided. The colorimeter includes one or more high power emitting diodes as the source of illuminating the sample. An elliptical mirror, with a central aperture to permit the light to penetrate on to the sample, directs the scattered light on to its focal point at a detector. The colorimeter includes a microprocessor which compares an output signal for the percent ratio value of the detected values of a sample and a standard sample. The colorimeter housing provides position sensors which allow the identification of the well being examined. Finally, the system is modified to measure cell motility by determining the ratio of motile sperms to total cell concentration.

Claims

1. A color measurement apparatus for determining the color composition on a specimen having an exposed surface, the apparatus comprising:

a first light emitting diode for producing light along a light path illuminating the specimen with light;

a sensing means along the light path for receiving light reflected from said specimen and producing a detection signal representative of the intensity of light reflected from the specimen in response thereto;

a mirror, elliptically shaped and having an aperture disposed therein, placed along the light path for focusing light reflected from the specimen and directing the reflected light to the sensing means; and

a processor for receiving the detection signal and determining the characteristic of the specimen in response thereto, by strobing the light source and a detector simultaneously.

2. The color measurement apparatus of claim 1, wherein the mirror consists of a dichroic mirror.

3. The color measurement apparatus of claim 1, wherein the specimen comprises semen.

4. The color measurement apparatus of claim 1, wherein the processor determines the characteristics as function of the ratio of the detected value of light reflected from the specimen and a standard value.

5. The color measurement apparatus of claim 1, further comprising a cover having a guiding slot for receiving a sample holder therein, and a plurality of position detectors for detecting the position of a sample, and wherein said sample holder includes a plurality of indexes such that said indexes match with the corresponding position detectors in said color measurement apparatus.

6. The color measurement of claim 5, further comprising:

an alignment detector, said alignment detector causing the first light and a second light to fire when a specimen is detected by the position detector,

a laser beam focusing on the specimen, and a sensing means including an apertured-mirror optical system to determine the light scattered from the specimen.