Protein with human choriogonadotropin inhibitory effect
First Claim
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1. A method for evaluating inhibition of human chorionic gonadotropin (hCG) production of a subject'"'"'s trophoblast cells comprising the steps of isolating a regulatory polypeptidea) which inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro;
- b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;
c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and
d) which exhibits a molecular weight of about 7,000 to about 10,000 daltons, as determined by ultrafiltration and gel exclusion chromatography;
from human decidual cells of said subject; and
quantifying the amount of the regulatory polypeptide produced in vitro by said subject'"'"'s human decidual cells.
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Abstract
A substance having an inhibitory effect on the production of human choriogonadotropin (hCG) is described, which comprises a protein isolated from human decidual cells with a preliminary amino acid analysis and a molecular weight of about 7,000 to about 10,000 daltons, and useful for use in inhibiting production of hCG in vivo or in vitro, may be measured as an indication of a cause of hCG inhibition, or used in the treatment of hCG-secreting tumors.
59 Citations
8 Claims
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1. A method for evaluating inhibition of human chorionic gonadotropin (hCG) production of a subject'"'"'s trophoblast cells comprising the steps of isolating a regulatory polypeptide
a) which inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro; -
b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;
c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and
d) which exhibits a molecular weight of about 7,000 to about 10,000 daltons, as determined by ultrafiltration and gel exclusion chromatography;
from human decidual cells of said subject; and
quantifying the amount of the regulatory polypeptide produced in vitro by said subject'"'"'s human decidual cells.- View Dependent Claims (2, 3, 7)
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4. A method for evaluating inhibition of chorionic gonadotropin (hCG) production of a subject'"'"'s trophoblast cells comprising the steps of isolating a regulatory polypeptide
a) which inhibits, in a dose dependent matter, the production of hCG by human trophoblasts in vitro; -
b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;
c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and
d) which exhibits a molecular weight of about 7,000 to about 10,000 Daltons, as determined by ultrafiltration and gel exclusion chromatography;
from human decidual cells of said subject; and
quantifying the amount of the regulatory polypeptide produced in vivo by said subject'"'"'s human decidual cells.- View Dependent Claims (5, 6, 8)
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Specification