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Method for predicting the efficacy of anti-cancer drugs

  • US 6,448,030 B1
  • Filed: 12/05/2000
  • Issued: 09/10/2002
  • Est. Priority Date: 02/18/2000
  • Status: Expired due to Fees
First Claim
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1. A method for predicting the efficacy of anti-cancer drugs on cells wherein both the cytotoxic and anti-migratory effects of the drug are measured, comprising the steps of:

  • a. disaggregating a biopsy specimen into individual cells to form a cell suspension;

    b. exposing said cells in said suspension to said anti-cancer drug;

    c. adding said exposed cells to the first chamber of a migration plate comprising two chambers separated by a porous membrane, said porous membrane being opaque to radiation;

    d. placing a chemical agent in the second chamber, said agent being capable of inducing migration of said cells from said first chamber to said second chamber;

    e. incubating cells to allow cell migration to occur;

    f. labeling cells on the side of the membrane closest to said second chamber with a fluorescent dye, said dye being capable of labeling only live cells;

    g. labeling cells on the side of the membrane closest to said first chamber with a fluorescent dye, said dye being capable of labeling only dead cells;

    h. stimulating the labeled cells on the side of the membrane closest to said second chamber with electromagnetic radiation of a first wavelength whereby said labeled cells will emit electromagnetic radiation of a second wavelength;

    i. measuring the emitted electromagnetic radiation from the side of the radiation opaque membrane closest to said second chamber;

    j. stimulating the labeled cells on the side of the membrane closest to said first chamber with electromagnetic radiation of a first wavelength whereby said labeled cells will emit electromagnetic radiation of a second wavelength; and

    k. measuring the emitted electromagnetic radiation from the side of the radiation opaque membrane closest to said first chamber, whereby the effect of a drug on said cell migration and said cell death is an indication of the effectiveness of said drug at arresting said cancer tissues.

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