Method for chemically acellularizing a biological tissue sample
First Claim
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1. A method for chemically acellularizing a peripheral nerve sample to produce an acellularized peripheral nerve construct, the method comprising:
- disrupting the cell membranes of the peripheral nerve sample by submersing the sample in a solution including glycerol; and
denaturing intracellular proteins within the cells of the sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce the acellularized peripheral nerve construct.
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Abstract
A method for chemically acellularizing a biological tissue sample, such as a peripheral nerve, is provided. The method includes disrupting the cell membranes of the biological tissue sample, and then denaturing intracellular proteins within the cells of the tissue sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce an acellularized tissue construct.
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Citations
21 Claims
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1. A method for chemically acellularizing a peripheral nerve sample to produce an acellularized peripheral nerve construct, the method comprising:
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disrupting the cell membranes of the peripheral nerve sample by submersing the sample in a solution including glycerol; and
denaturing intracellular proteins within the cells of the sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce the acellularized peripheral nerve construct. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for producing a chemically acellularized peripheral nerve construct, the method comprising:
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harvesting a peripheral nerve sample from a donor;
submersing the peripheral nerve sample in a glycerol solution for disrupting the cell membranes of the peripheral nerve; and
submersing the peripheral nerve sample in at least one detergent solution for denaturing the intracellular proteins of the cells of the peripheral nerve and removing the denatured proteins while leaving the extracellular matrix intact to produce the acellularized peripheral nerve construct without using mechanical agents. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for producing a chemically acellularized peripheral nerve construct, comprising:
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harvesting a peripheral nerve segment from a donor;
disrupting the cell membranes and removing lipid-soluble cell structures of the peripheral nerve segment using a glycerol solution;
denaturing intracellular proteins within the cells of the peripheral nerve segment using at least one ionic detergent; and
removing the denatured proteins from the cells while leaving the extracellular matrix intact using ionic and nonionic detergents to produce the acellularized peripheral nerve construct without using mechanical agents. - View Dependent Claims (21)
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Specification