Antibiotics and methods of using the same

• US 6,448,224 B1
• Filed: 05/06/1998
• Issued: 09/10/2002
• Est. Priority Date: 05/06/1998
• Status: Expired due to Term

• Current Assignee: St Jude Children's Research Hospital Incorporated (Abbott Laboratories)
• Original Assignee: St Jude Children's Research Hospital Incorporated (Abbott Laboratories)
• Inventors: Novak, Rodger, Tuomanen, Elaine I.
• Primary Examiner(s): BUGAISKY, GABRIELE E
• Application Number:
  US09/073,541
• Time in Patent Office: 1,588 Days
• Field of Search: 530/300, 530/324, 530/325, 530/326, 530/350, 514/12, 514/2, 514/13, 514/14
• US Class Current: 514/2.4
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/3156 from Streptococcus pneumoniae (Pneumococcus) Streptokinase C07K14/3153
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68
• CPC Class Codes: C12N 9/1223 Phosphotransferases with a nitrogenous group as acceptor (2.7.3)
• CPC Class Codes: C12N 9/14 Hydrolases (3)
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention discloses novel antibiotic peptides, including naturally occurring peptides. The present invention also includes the nucleic acid sequence encoding such peptides and the corresponding amino acid sequences. Methods of identifying, making, and using the antibiotic peptides are also disclosed.

Claims

1. A peptide comprising the amino acid sequence of SEQ ID NO:

2.

2. The peptide of claim 1 wherein the peptide contains to 35 amino acids.

7. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 1 and a pharmaceutically acceptable carrier.

20. A fusion or chimeric peptide comprising the peptide of claim 1.

3. A peptide that consists of the amino acid sequence of SEQ ID NO:

2.

4. A peptide comprising the amino acid sequence of SEQ ID NO:

4.

5. The peptide of claim 4 wherein the peptide contains to 35 amino acids.

14. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 4 and a pharmaceutically acceptable carrier.

21. A fusion or chimeric peptide comprising the peptide of claim 4.

6. A peptide that consists of the amino acid sequence of SEQ ID NO:

4.

8. A peptide comprising the amino acid sequence of SEQ ID NO:

2 with one conservative amino acid substitution;

wherein the peptide can inhibit the growth or kill both the wild type strain of bacteria and a strain of bacteria that either is missing or has a defective autolysin;

wherein the replacement of an amino acid residue of SEQ ID NO;

2 by a substitute amino acid residue constitutes a conservative amino acid substitution when both the amino acid residue and the substitute amino acid residue are members of the same class selected from the group consisting of nonpolar amino acids, aromatic amino acids, polar neutral amino acids, positively charged amino acids and negatively charged amino acids.

19. A fusion or chimeric peptide comprising the peptide of claim 8.

24. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 8 and a pharmaceutically acceptable carrier.

9. A peptide comprising the amino acid sequence of SEQ ID NO:

4 with one conservative amino acid substitution;

wherein the peptide can inhibit the growth or kill both the wild type strain of bacteria and a strain of bacteria that either is missing or has a defective autolysin;

wherein the replacement of an amino acid residue of SEQ ID NO;

4 by a substitute amino acid residue constitutes a conservative amino acid substitution when both the amino acid residue and the substitute amino acid residue are members of the same class selected from the group consisting of nonpolar amino acids, aromatic amino acids, polar neutral amino acids, positively charged amino acids and negatively charged amino acids.

18. A fusion or chimeric peptide comprising the peptide of claim 9.

23. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 9 and a pharmaceutically acceptable carrier.

10. A peptide comprising the amino acid sequence of SEQ ID NO:

44.

11. The peptide of claim 10 wherein the peptide contains 27 to 35 amino acids.

15. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 10 and a pharmaceutically acceptable carrier.

17. A fusion or chimeric peptide comprising the peptide of claim 10.

12. A peptide comprising the amino acid sequence of SEQ ID NO:

44 with one conservative amino acid substitution;

wherein the peptide can inhibit the growth or kill both the wild type strain of bacteria and a strain of bacteria that either is missing or has a defective autolysin;

wherein the replacement of an amino acid residue of SEQ ID NO;

44 by a substitute amino acid residue constitutes a conservative amino acid substitution when both the amino acid residue and the substitute amino acid residue are members of the same class selected from the group consisting of nonpolar amino acids, aromatic amino acids, polar neutral amino acids, positively charged amino acids and negatively charged amino acids.

16. A fusion or chimeric peptide comprising the peptide of claim 12.

22. A pharmaceutical composition for treating a bacterial infection comprising the peptide of claim 12 and a pharmaceutically acceptable carrier.

13. A peptide that consists of the amino acid sequence of SEQ ID NO:

44.