Cellulase for use in industrial processes

• US 6,451,063 B1
• Filed: 09/25/1996
• Issued: 09/17/2002
• Est. Priority Date: 09/25/1996
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Winetzky, Deborah, Swanson, Barbara, Clarkson, Kathleen A.
• Primary Examiner(s): DIAMOND, ALAN D
• Application Number:
  US08/719,506
• Time in Patent Office: 2,183 Days
• Field of Search: 252/8.91, 510/320, 510/321, 510/392, 510/393, 510/530, 426/623, 426/648, 810/2, 813/7, 162/72, 162/91, 162/4, 162/5, 162/158, 435/263, 435/267, 435/277
• US Class Current: 8/102
• CPC Class Codes: A23K 10/14 Pretreatment of feeding-stuffs with enzymes
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: D06M 16/003 with enzymes or microorganisms
• CPC Class Codes: D06M 2101/06 cellulosic
• CPC Class Codes: D06P 5/02 After-treatment
• CPC Class Codes: D06P 5/137 with other compounds
• CPC Class Codes: D06P 5/158 with other compounds

Abstract

A method for treating cellulosic materials is disclosed which comprises contacting the cellulosic material with a cellulase obtainable from Thermomonospora fusca corresponding to E5 or a derivative thereof. Particularly preferred methods comprise stonewashing and detergent cleaning of cotton fabrics, the production of paper products, as an additive to animal feed and in the production of food, starch, ethanol and sugar.

Claims

1. A method of treating cellulosic material comprising contacting said material with a cellulase obtainable from Thermomonospora fusca corresponding to E5, a truncated E5, or a derivative thereof wherein said contacting takes place at a pH greater than 7.0 but less than about 10.0.

2. The method according to claim 1, wherein said truncated E5 is obtained through proteolytic cleavage.

3. The method according to claim 1, wherein said truncated E5 is obtained through genetic engineering techniques.

4. The method according to claim 1, wherein said cellulosic material comprises cellulose containing fabric.

5. The method according to claim 4, wherein said cellulose containing fabric is treated so as to effect a stonewashed effect.

6. The method according to claim 4, wherein said cellulose containing fabric is treated so as to improve the appearance and/or feel of said fabric.

7. The method according to claim 4, wherein said cellulase is incorporated into a detergent composition.

8. The method according to claim 6, wherein said cellulose containing fabric comprises a faded, colored fabric and said improvement comprises improving the appearance of said faded colored fabric by rejuvenating the color thereof.

9. The method according to claim 6, wherein the improvement comprises a softer or smoother feel to said fabric.

10. The method according to claim 1, wherein said cellulosic material comprises wood pulp.

11. The method according to claim 1, wherein said cellulosic material comprises animal feed or grain.