Identification of disease predictive nucleic acids
First Claim
1. A method of identifying a target nucleic aid sequence, said nucleic acid sequence being predictive of a preselected disease state in cells containing the nucleic acid sequence comprising:
- comparing members of a set of mRNA alternative transcript forms from a common gene, said alternative transcript forms being transcribed from identical DNA, said gene being predictive of said disease state in cells containing the gene;
identifying at least one molecular interaction site from among those present in said members of the set based on secondary structure of said mRNA alternative transcript forms;
said molecular interaction site being present in cells likely to have said disease state; and
ascertaining said target nucleic acid sequence from said molecular interaction site.
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Abstract
The present invention is directed to methods of identifying target nucleic acid sequences which are predictive of preselected disease states or biological conditions in cells containing the nucleic acid sequence. Members of a set of mRNA molecules from a common gene, but containing different sequences and structures, are compared. The gene is predictive of the disease state or biological condition in cells containing the gene. At least one molecular interaction site from among those present in the members of the set are identified. The molecular interaction site is present in cells likely to have the disease state or biological condition. At least one nucleic acid sequence from the molecular interaction site is ascertained.
27 Citations
9 Claims
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1. A method of identifying a target nucleic aid sequence, said nucleic acid sequence being predictive of a preselected disease state in cells containing the nucleic acid sequence comprising:
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comparing members of a set of mRNA alternative transcript forms from a common gene, said alternative transcript forms being transcribed from identical DNA, said gene being predictive of said disease state in cells containing the gene;
identifying at least one molecular interaction site from among those present in said members of the set based on secondary structure of said mRNA alternative transcript forms;
said molecular interaction site being present in cells likely to have said disease state; and
ascertaining said target nucleic acid sequence from said molecular interaction site. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification
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Current AssigneeIonis Pharmaceuticals, Inc.
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Original AssigneeISIS Pharmaceuticals Incorporated
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InventorsEcker, David J.
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Primary Examiner(s)Fredman, Jeffrey
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Assistant Examiner(s)GOLDBERG, JEANINE ANNE
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Application NumberUS09/200,355Time in Patent Office1,392 DaysField of Search435/6, 435/375, 536/23.1, 536/24.31US Class Current435/6.11CPC Class CodesC12Q 1/6809 Methods for determination o...
