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Methods and compositions for polypeptide engineering

  • US 6,455,253 B1
  • Filed: 04/27/2000
  • Issued: 09/24/2002
  • Est. Priority Date: 12/18/1996
  • Status: Expired due to Fees
First Claim
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1. A method for evolving a polypeptide to comprise an improved serum half-life or stability, the polypeptide being encoded by a DNA substrate molecule, the method comprising:

  • (a) providing a library of variants of the DNA substrate molecule;

    (b) transforming the variants of (a) into host cells;

    (c) expressing the variants of (b) to provide polypeptide expression products;

    (d) screening or selecting from the polypeptide expression products of (c) an evolved polypeptide comprising the improved serum half-life or stability relative to the polypeptide encoded by the DNA substrate molecule in vivo;

    (e) recovering an evolved DNA substrate molecule that encodes the evolved polypeptide of (d); and

    , (f) expressing the evolved DNA substrate molecule, thereby providing the evolved polypeptide comprising the improved serum half-life or stability.

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