Fabrication of vascularized tissue

• US 6,455,311 B1
• Filed: 04/28/2000
• Issued: 09/24/2002
• Est. Priority Date: 04/30/1999
• Status: Expired due to Fees

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Vacanti, Joseph P.
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/560,480
• Time in Patent Office: 879 Days
• Field of Search: 435/1--, 435/395
• US Class Current: 435/395
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/44 Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3886 comprising two or more cell types
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: B33Y 70/00 Materials specially adapted for additive manufacturing
• CPC Class Codes: C12N 2502/14 hepatocytes
• CPC Class Codes: C12N 2502/28 Vascular endothelial cells
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0671 Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
• CPC Class Codes: C12N 5/0697 Artificial constructs associating cells of different lineages, e.g. tissue equivalents blood vessels C12N5/0691

Abstract

Disclosed and claimed is a laminar structure. The laminar structure has multiple layers. Each layer has tissue and vasculature. The layers are abjacent. The vasculature is in three dimensions through the structure. The structure has connections for flow into and out of the vasculature. The structure can be implanted directly by connecting blood vessels to flow into and out of the vasculature.

Claims

1. A laminated structure consisting essentially of multiple layers, wherein each layer consists essentially of tissue and vasculature, wherein the layers are assembled adjacent to each other by folding and compacting, the resulting vasculature is in three dimensions throughout the structure, and the structure has connections for flow into and out of the vasculature, and whereby the structure can be implanted directly by connecting blood vessels to flow into and out of the vasculature.

2. The structure of claim 1 wherein the vasculature comprises endothelial cells.

3. The structure of claim 2 further comprising cells selected from the group consisting of parenchymal cells, cells forming cartilage or bone, muscle cells, and nerve cells.

4. The structure of claim 3 wherein the parenchymal cells are derived from organs selected from the group consisting of heart, liver, pancreas, intestine, brain, kidney, reproductive tissues and lung.

5. The structure of claim 1 comprising glomerular tissue and a bile duct drainage system.

6. The structure of claim 1 comprising alveolar or airway tissue.

7. The structure of claim 1 comprising nerves or lymphatics.