Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates

• US 6,458,355 B1
• Filed: 07/24/1998
• Issued: 10/01/2002
• Est. Priority Date: 01/22/1998
• Status: Expired due to Fees

• Current Assignee: Genentech, Inc. (Roche Holding AG)
• Original Assignee: Genentech, Inc. (Roche Holding AG)
• Inventors: Shahrokh, Zahra, Koumenis, Iphigenia, Leong, Steven, Hsei, Vanessa, Zapata, Gerardo, Presta, Leonard
• Primary Examiner(s): Mertz, Prema
• Assistant Examiner(s): Hamud, Fozia
• Application Number:
  US09/121,952
• Time in Patent Office: 1,530 Days
• Field of Search: 424/145.1, 424/133.1, 424/134.1, 424/141.1, 424/158.1, 424/178.1, 424/280.1, 530/387.3, 530/388.1, 530/388.23, 530/866
• US Class Current: 424/145.1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: C07K 16/244 Interleukins [IL]
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/54 F(ab')2
• CPC Class Codes: C07K 2317/55 Fab or Fab'
• CPC Class Codes: C07K 2317/565 Complementarity determining region [CDR]
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: Y10S 530/866 involving immunoglobulin or antibody fragment, e.g. fab', fab, fv, fc, heavy chain or light chain

Abstract

Provided are methods for treating inflammatory diseases in a patient comprising administering to the patient an effective amount of a conjugate consisting essentially of one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein at least one antibody fragment comprises an antigen binding site that binds to human IL-8, and wherein the apparent size of the conjugate is at least about 500 kD.

Claims

1. A method of treating an inflammatory disorder in a mammal comprising administering to the mammal an effective amount of a conjugate of a single antibody fragment covalently attached to 1 or 2 polyethylene glycol (PEG) molecules, wherein the antibody fragment is a F(ab′

)₂ comprising (1) a first chain that is either a light chain or a heavy chain (2) a first opposite chain that is either a heavy chain opposite the first light chain or a light chain opposite the first heavy chain (3) a second chain that is either a light chain or a heavy chain and (4) a second opposite chain that is either a heavy chain opposite the second light chain or a light chain opposite the second heavy chair, wherein every PEG molecule is covalently attached to a first cysteine residue in the first or second chain that would ordinarily form a disulfide bridge with a second cysteine residue in the first or second opposite chain, wherein the disulfide bridge is avoided by substitution of another amino acid residue for the second cysteine residue in the first or second opposite chain, wherein the F(ab′

)₂ comprises an antigen binding site that binds to human interleukin-8 (IL-8), and wherein the apparent size of the conjugate is at least about 500 kD.

2. The method of claim 1 wherein the inflammatory disorder is an ischemic reperfusion disorder.

3. The method of claim 1, wherein the inflammatory disorder is hypovolemic shock.

4. The method of claim 2 wherein the ischemic reperfusion disorder is a surgical tissue reperfusion injury.

5. The method of claim 2, wherein the ischemic reperfusion disorder is myocardial ischemia.

6. The method of claim 2, wherein the ischemic reperfusion disorder is acute myocardial infarction.

7. The method of claim 1, wherein the apparent size of the conjugate is at least about 800 kD.

8. The method of claim 7, wherein the apparent size of the conjugate is at least about 1,400 kD.

9. The method of claim 1, wherein at least one PEG has an average molecular weight of at least about 20 kD.

10. The method of claim 9, wherein the PEG has an average molecular weight of at least about 30 kD.

11. The method of claim 10, wherein the PEG has an average molecular weight of at least about 40 kD.

12. The method of claim 9, wherein the PEG is a single chain molecule.

13. The method of claim 11, wherein the PEG is a branched chain molecule.

14. The method of claim 11, wherein the antibody fragment comprising the anti-human IL-8 antigen binding site is monoclonal and humanized.

15. The method of claim 14, wherein the anti-human IL-8 antigen binding site comprises the complementarity determining regions of a light chain polypeptide amino acid sequence selected from the group consisting of the 6G4V11N35A light chain polypeptide amino acid sequence of SEQ ID NO:

56 and the 6G4V11N35E light chain polypeptide amino acid sequence of SEQ ID NO;

62.

16. The method of claim 1, wherein the covalent structure of the conjugate is free of any matter other than the antibody fragment and PEG molecules that form the conjugate.

17. The method of claim 1 wherein the mammal is human.

18. The method of claim 1, wherein the second cysteine residue is substituted with a serine residue in the first or second opposite chain.

19. A method of treating an inflammatory disorder in a mammal comprising administering to the mammal an effective amount of a conjugate of a single antibody fragment covalently attached to a single polyethylene glycol (PEG) molecule, wherein the antibody fragment is a F(ab′

)₂ comprising (1) a first chain that is either a light chain or a heavy chain (2) a first opposite chain that is either a heavy chain opposite the first light chain or a light chain opposite the first heavy chain (3) a second chain that is either a light chain or a heavy chain and (4) a second opposite chain that is either a heavy chain opposite the second light chain or a light chain opposite the second heavy chain, wherein every PEG molecule is covalently attached to a first cysteine residue in the first or second chain that would ordinarily form a disulfide bridge with a second cysteine residue in the first or second opposite chain, wherein the disulfide bridge is avoided by substitution of another amino acid residue for the second cysteine residue in the first or second opposite chain, wherein the F(ab′

)₂ comprises an antigen binding site that binds to human interleukin-8 (IL-8), and wherein the apparent size of the conjugate is at least about 500 kD.

20. The method of claim 19, wherein the inflammatory disorder is an ischemic reperfusion disorder.

21. The method of claim 20, wherein the ischemic reperfusion disorder is hypovolemic shock.

22. The method of claim 20, wherein the ischemic reperfusion disorder is surgical tissue reperfusion injury.

23. The method of claim 20, wherein the ischemic reperfusion disorder is myocardial ischemia.

24. The method of claim 20, wherein the ischemic reperfusion disorder is acute myocardial infarction.

25. The method of claim 19, wherein the antibody fragment is humanized.

26. The method of claim 25, wherein the anti-human IL-8 antigen binding site comprises the complementarity determining regions of a light chain polypeptide amino acid sequence selected from the group consisting of the 6G4V11N35A light chain polypeptide amino acid sequence of SEQ ID NO:

56 and the 6G4V11N35E light chain polypeptide amino acid sequence of SEQ ID NO;

62.

27. The method of claim 19, wherein the covalent structure of the conjugate is free of any matter other than the antibody fragment and PEG molecules that form the conjugate.

28. The method of claim 19, wherein the mammal is human.

29. The method of claim 19, wherein the second cysteine residue is substituted with a serine residue in the first opposite chain.

30. The method of claim 20, wherein the PEG has an average molecular weight of at least about 20 kD.

31. The method of claim 30, wherein the PEG has an average molecular weight of at least about 30 kD.

32. The method of claim 31, wherein the PEG has an average molecular weight of at least about 40 kD.

33. The method of claim 30, wherein the PEG is a single chain molecule.

34. The method of claim 32, wherein the PEG is a branched chain molecule.