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Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable

  • US 6,458,584 B1
  • Filed: 03/03/1999
  • Issued: 10/01/2002
  • Est. Priority Date: 12/23/1996
  • Status: Expired due to Term
First Claim
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1. A method for using a reuseable microchip to identify at least one nucleic acid sequence in nucleic acids of a sample, said method comprising:

  • (a) providing a customized matrix of oligonucleotides on the microchip in an arrangement that is an ordered scheme designed to present a detectable pattern as a whole that identifies the sequences of nucleic acids in the sample;

    (b) hybridizing nucleic acids from the sample on said microchip; and

    (c) identifying at least one nucleic acid sequence in said sample by the pattern detected as a whole on the microchip, said pattern resulting from the locations of oligonucleotides which hybridized to the sample nucleic acids wherein the customized matrix of oligonucleotides on the reusable microchip is formed by a plurality of gel elements, wherein the number of elements is determined by the number of oligonucleotides in the matrix and wherein each gel element contains one oligonucleotide of a desired nucleic acid sequence length and concentration, each gel element being separated from another by interstitial spaces, said oligonucleotides being positioned in specific locations and wherein said matrix comprises oligonucleotides comprising the base sequences;

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