Test piece and system for reading out image information from the test piece

• US 6,458,601 B1
• Filed: 05/16/2000
• Issued: 10/01/2002
• Est. Priority Date: 05/18/1999
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Kimura, Toshihito
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): Padmanabhan, Kartic
• Application Number:
  US09/572,886
• Time in Patent Office: 868 Days
• Field of Search: 422/50, 422/68.1, 435/6, 435/7.1, 435/7.2, 435/7.4, 435/7.5, 435/7.6, 435/7.7, 435/7.71, 435/7.72, 435/7.8, 435/7.9, 435/91.1, 435/91.2, 435/810, 435/7.92, 435/174, 435/176, 435/177, 435/181, 435/287.1, 435/287.2, 435/287.8, 435/287.9, 435/288.3, 435/288.7, 435/808, 435/969.973, 436/501, 436/164, 436/518, 436/524, 436/528, 436/805
• US Class Current: 436/518
• CPC Class Codes: B01L 2300/0893 having a very large number of wells, microfabricated wells
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/969 Multiple layering of reactants
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

A test piece for use in biological analyses includes a plurality of different known specific binding substances disposed in predetermined positions on a substrate. The specific binding substances are disposed on a plurality of surfaces provided by the substrate and arranged in the direction of thickness of the substrate.

Claims

1. A test piece for use in biological analyses comprising:

a planar substrate having an upper surface and a lower surface;

a plurality of different known specific binding substances disposed in predetermined positions on said substrate;

wherein the specific binding substances are disposed on said upper surface and on said lower surface of said substrate so as not to be within said substrate, the specific binding substances being disposed such that they do not contact one another on each said upper surface and said lower surface, and

wherein the specific binding substances are disposed on said upper surface and on said lower surface of said substrate such that the specific binding substances on said upper surface do not have the specific binding substances on said lower surface disposed directly beneath them when said test piece is viewed in a direction of thickness of said substrate.

3. The test piece of claim 1, wherein said planar substrate is positioned between the specific binding substances.

5. The test piece of claim 1, wherein said planar substrate is optically transparent.

2. A test piece for use in biological analyses comprising:

a plurality of planar substrates each having an upper surface and a lower surface, said substrates being stacked and bonded together in a vertical direction with a spacer disposed between the substrates;

a plurality of different known specific binding substances disposed in predetermined positions on said upper surface of each of said plurality of substrates so as not to be within any individual one of said plurality of substrates;

wherein the specific binding substances are disposed such that they do not contact one another on said upper surface of each of said plurality of substrates; and

wherein the specific binding substances are disposed such that the specific binding substances on one of said plurality of substrates are not disposed directly beneath the specific binding substances on another of said plurality of substrates when said test piece is viewed in a direction of thickness of said substrate.

6. The test piece of claim 2, wherein said planar substrate is optically transparent.

4. A test piece for use in biological analyses comprising:

a plurality of planar substrates each having an upper surface and a lower surface, said substrates being stacked and bonded together in a vertical direction with a spacer disposed between the substrates, such that the stacked substrates are parallel to one another;

a plurality of different known specific binding substances disposed in predetermined positions on said upper surface of each of said plurality of substrates so as not to be within any individual one of said plurality of substrates;

wherein the specific binding substances are disposed such that they do not contact one another on said upper surface of each of said plurality of substrates; and

wherein the specific binding substances are disposed such that the specific binding substances on one of said plurality of substrates are not disposed directly beneath the specific binding substances on another of said plurality of substrates when said test piece is viewed in a direction of thickness of said substrate.