Method for analyzing polynucleotides

• US 6,458,945 B1
• Filed: 11/09/2000
• Issued: 10/01/2002
• Est. Priority Date: 10/01/1998
• Status: Expired due to Term

• Current Assignee: Agena Bioscience Inc. (Mesa Laboratories Inc.)
• Original Assignee: Variagenics, Inc. (Arca Biopharma, Inc.)
• Inventors: Verdine, Gregory L., Stanton, Vincent P. Jr., Wolfe, Jia Liu, Kawate, Tomohiko
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US09/709,596
• Time in Patent Office: 691 Days
• Field of Search: 435/6, 435/91.1, 435/91.21, 536/23.1, 536/25.3, 536/25.32
• US Class Current: 536/25.3
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 19/14 Pyrrolo-pyrimidine radicals
• CPC Class Codes: C07H 19/20 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12N 15/11 DNA or RNA fragments; Modified forms thereof DNA or RNA not used in recombinant technology, C07H21/00; Non-coding nucleic acids having a biological activity
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6872 involving mass spectrometry
• CPC Class Codes: C12Q 2523/107 Chemical cleaving agents
• CPC Class Codes: C12Q 2525/101 incorporating non-naturally occurring nucleotides, e.g. inosine
• CPC Class Codes: C12Q 2565/627 being a mass spectrometer

Abstract

The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.

Claims

1. A method for synthesizing a polynucleotide comprising mixing a compound having the chemical structure:

wherein R¹ is selected from the group consisting of;

with adenosine triphosphate, guanosine triphosphate, and thymidine or uridine triphosphate in the presence of one or more polymerases.

2. A method for synthesizing a polynucleotide comprising mixing a compound having the chemical structure:

wherein R¹ is selected from the group consisting of;

with adenosine triphosphate, cytidine triphosphate and guanosine triphosphate in the presence of one or more polymerases.

3. A method for synthesizing a polynucleotide, comprising mixing a compound having the chemical structure:

wherein R¹ is selected from the group consisting of;

with cytidine triphosphate, guanosine triphosphate, and thymidine triphosphate in the presence of one or more polymerases.

4. A method for synthesizing a polynucleotide, comprising mixing a compound having the chemical structure:

wherein R¹ is selected from the group consisting of;

with adenosine triphosphate, cytidine triphosphate and thymidine triphosphate in the presence of one or more polymerases.