Caffeine detector

• US 6,461,873 B1
• Filed: 03/17/2000
• Issued: 10/08/2002
• Est. Priority Date: 03/18/1999
• Status: Expired due to Fees

• Current Assignee: Catania Enterprises, Inc.
• Original Assignee: Catania Enterprises, Inc.
• Inventors: Baugh, Steven, Catania, Daniel, Ignelzi, Steven
• Primary Examiner(s): NGUYEN, BAO THUY L
• Application Number:
  US09/528,194
• Time in Patent Office: 935 Days
• Field of Search: 436/518, 436/20, 436/514, 436/525, 436/810, 436/816, 436/901, 422/55, 422/56, 422/57, 422/58, 422/68.1, 422/102, 435/7.1, 435/7.93, 435/7.94, 435/970, 435/287.2, 435/973, 435/288.4, 435/288.7, 435/975
• US Class Current: 436/518
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/946 CNS-stimulants, e.g. cocaine, amphetamines
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine

Abstract

The caffeine detector uses a paper chromatographic technique to measure the concentration of caffeine in a beverage. The device includes a well or other receptacle for containing a predetermined test volume of the beverage. A paper strip is suspended so that one end of the strip dips into the well. The strip is divided into two zones, the first zone being coated with at least one molecular imprint polymer (MIP) which absorbs substances which may interfere with quantification of caffeine. The second zone is coated with an MIP which selectively absorbs caffeine and chromogenic reagents which provide calorimetric visualization of the migration of caffeine through the second zone. The second zone bears indicia calibrating the paper strip so that the concentration of caffeine in the beverage may be determined by the height or distance which the caffeine migrates up the paper strip.

Claims

1. A caffeine detector for detecting the presence and concentration of caffeine in a liquid, the detector comprising an elongated strip of absorbent paper having:

a) a first zone having a stationary phase disposed thereon including a first molecular imprint polymer for selectively absorbing compounds interfering with the detection of caffeine; and

b) a second zone having a stationary phase disposed thereon including a second molecular imprint polymer for selectively absorbing caffeine and at least one chromogenic agent which produces a color change in the presence of caffeine; and

c) said paper strip being suspended above a predetermined volume of a liquid with a portion of the first zone dipping into the liquid, the presence and concentration of caffeine in the liquid is determined by the height of the color change produced in the second zone.

2. The caffeine detector according to claim 1, further comprising a plurality of indicia imprinted on said paper strip in the second zone calibrated to correspond to the caffeine concentration in a predetermined volume of a liquid being analyzed.

3. The caffeine detector according to claim 1, wherein said first molecular imprint polymer consists essentially of a polymer polymerized from methacrylic acid cross-linked with ethylene glycol dimethacrylate in the presence of theobromine and a porogen, said polymer having a capacity for selectively absorbing theobromine.

4. The caffeine detector according to claim 3, wherein said first molecular imprint polymer has a particle size less than one hundred eighty micrometers.

5. The caffeine detector according to claim 3, wherein said first molecular imprint polymer has a particle size less than thirty-eight micrometers.

6. The caffeine detector according to claim 1, wherein said second molecular imprint polymer consists essentially of a polymer polymerized from methacrylic acid cross-linked with ethylene glycol dimethacrylate in the presence of caffeine and a porogen, said polymer having a capacity for selectively absorbing caffeine.

7. The caffeine detector according to claim 6, wherein said second molecular imprint polymer has a particle size less than one hundred eighty micrometers.

8. The caffeine detector according to claim 6, wherein said second molecular imprint polymer has a particle size less than thirty-eight micrometers.

9. The caffeine detector according to claim 1, further comprising a base platform having a well defined therein, said paper strip being suspended above said base platform with a portion of the first zone dipping into the well.

10. The caffeine detector according to claim 9, wherein said well is calibrated to contain a predetermined volume of a liquid for analysis.

11. The caffeine detector according to claim 9, wherein said well is calibrated to contain about two milliliters of a liquid for analysis.

12. The caffeine detector according to claim 9, further comprising:

a) a vertical wall attached to said base platform; and

b) a support wall attached to said vertical wall and extending parallel to and above said base platform, the support wall having a slot defined therein aligned above said well, said paper strip being inserted through said slot with a portion of the first zone dipping into said well.