Method for calibration and measurement in a micro-dialysis system, and a micro-dialysis system

• US 6,463,394 B1
• Filed: 10/06/1999
• Issued: 10/08/2002
• Est. Priority Date: 10/07/1998
• Status: Expired due to Term

• Current Assignee: Sphere Medical Limited (Sphere Medical Holding Ltd.)
• Original Assignee: Siemens Elema AB
• Inventors: Von Bahr, Pontus
• Primary Examiner(s): Hoff, Marc S.
• Assistant Examiner(s): Charioui, Mohamed
• Application Number:
  US09/413,024
• Time in Patent Office: 1,098 Days
• Field of Search: 702/100, 702/55, 702/34, 210/87, 210/646, 210/96.2, 210/143, 210/97, 210/321.71, 210/321.79, 210/645, 210/739, 73/10.6, 700/83, 600/341, 600/578, 600/583, 600/347, 435/6, 435/183
• US Class Current: 702/100
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes

Abstract

In a method for calibration and measurement of a micro-dialysis system and a micro-dialysis system, one sensor is employed. Measurement times in micro-dialysis tend to be long, since concentration equilibrium at the measurement probe is necessary. The method enables calibration and measurement to be performed with two dialysates with differing analyte contents in order to facilitate measurement with no need to wait for complete equilibrium.

Claims

1. A method for measurement in a micro-dialysis system, comprising:

supplying a first dialysate, containing an analyte at a first concentration, for exposure to a substance to be measured;

performing a measurement on said first dialysate before and after exposure to said substance to be measured to obtain a first calibration reading and a first measurement reading respectively;

supplying a second dialysate, containing an analyte at a second concentration, for exposure to said substance to be measured;

performing a measurement on said second dialysate before and after exposure to said substance to be measured to obtain a second calibration reading and a second measurement reading respectively; and

determining said concentration of said substance from said first and second concentrations, said first and second calibration readings, and said first and second measurement readings.

2. A method as claimed in claim 1 comprising supplying said first and second dialysates to said substance in a predetermined pattern and preceding each measurement reading with no more than one of said calibration readings respectively obtained using one of said dialysates.

3. A method as claimed in claim 1 comprising successively alternatingly supplying said first dialysate and said second dialysate to said substance.

4. A method for measurement in a micro-dialysis system, comprising:

providing a measurement probe;

providing a sensor, connected to said measurement probe, for conducting a measurement;

supplying a first dialysate, containing an analyte at a first concentration, to said sensor and to said measurement probe;

obtaining a first calibration reading by measuring a concentration of said first dialysate in said sensor while exposing said first dialysate in said measurement probe to a substance to be measured;

transferring said first dialysate in said measurement probe, after exposure to said substance, to said sensor and measuring a concentration of said substance in said first dialysate to obtain a first measurement reading from said sensor;

supplying a second dialysate, containing an analyte at a second concentration, to said sensor and to said measurement probe;

obtaining a second calibration reading by measuring a concentration of said second dialysate in said sensor while exposing said second dialysate in said measurement probe to said substance;

transferring said second dialysate in said measurement probe, after exposure to said substance, to said sensor and measuring a concentration of said substance in said first dialysate to obtain a second measurement reading from said sensor; and

determining said concentration of said substance from said first and second concentrations, said first and second calibration readings and said first and second measurement readings.

5. A micro-dialysis system comprising:

means for supplying a first dialysate, containing an analyte at a first concentration, for exposure to a substance to be measured;

means for performing a measurement on said first dialysate before and after exposure to said substance to be measured to obtain a first calibration reading and a first measurement reading respectively;

means for supplying a second dialysate, containing an analyte at a second concentration, for exposure to said substance to be measured;

means for performing a measurement on said second dialysate before and after exposure to said substance to be measured to obtain a second calibration reading and a second measurement reading respectively; and

means for determining said concentration of said substance from said first and second concentrations, said first and second calibration readings and said first and second measurement readings.