Trace level detection of analytes using artificial olfactometry

• US 6,467,333 B2
• Filed: 03/22/2001
• Issued: 10/22/2002
• Est. Priority Date: 06/19/1998
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Wong, Bernard, Severin, Erik J., Lewis, Nathan S.
• Primary Examiner(s): Williams, Herzon
• Assistant Examiner(s): WIGGINS, JOHN DAVID
• Application Number:
  US09/815,618
• Publication Number:
  US 20020017125A1
• Time in Patent Office: 579 Days
• Field of Search: 73/310.5, 73/232, 73/233, 73/233.4, 73/614.1, 73/530.1, 422/84, 422/88, 422/78, 422/101, 600/530, 600/531, 600/532
• US Class Current: 73/31.05
• CPC Class Codes: A61B 5/00 Measuring for diagnostic purposes radiation diagnosis A61B6/00; diagnosis by ultrasonic, sonic or infrasonic waves A61B8/00; Identification of persons
• CPC Class Codes: A61B 5/082 Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath A61B5/083, A61B5/091 take precedence
• CPC Class Codes: A61B 5/4547 Evaluating teeth
• CPC Class Codes: G01N 1/22 in the gaseous state specially adapted for biological material G01N33/497; measuring breath flow A61B5/087
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 33/0031 comprising two or more sensors, e.g. a sensor array electrochemical electrode arrays G01N27/27
• CPC Class Codes: G01N 33/0034 comprising neural networks or related mathematical techniques
• CPC Class Codes: G01N 33/0044 for H2S, sulfides
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: Y10S 436/90 Breath testing
• CPC Class Codes: Y10S 977/879 Material
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/200833 Carbonyl, ether, aldehyde or ketone containing
• CPC Class Codes: Y10T 436/212 Aromatic
• CPC Class Codes: Y10T 436/214 Acyclic [e.g., methane, octane, isoparaffin, etc.]
• CPC Class Codes: Y10T 436/216 Unsaturated [e.g., ethylene, diene, etc.]

Abstract

The present invention provides a device for detecting the presence of an analyte, such as for example, a lightweight device, including: a sample chamber having a fluid inlet port for the influx of the analyte; a fluid concentrator in flow communication with the sample chamber wherein the fluid concentrator has an absorbent material capable of absorbing the analyte and capable of desorbing a concentrated analyte; and an array of sensors in fluid communication with the concentrated analyte to be released from the fluid concentrator.

Claims

1. A device for detecting the presence of an analyte, wherein said analyte is a microorganism marker gas indicative of a respiratory tract infection, said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said analyte;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said analyte and capable of desorbing a concentrated analyte; and

c) an array of sensors in fluid communication with said concentrated analyte.

2. The device according to 1, claim further comprising a detector operatively associated with each sensor and providing a response in the presence of said analyte.

3. A device in accordance with claim 1, wherein said fluid concentrator is disposed within said sample chamber.

4. A device in accordance with claim 1, wherein said sensor array is disposed within said sample chamber.

5. A device in accordance with claim 1, wherein said fluid concentrator and said sensor array are disposed within said sample chamber.

6. A device in accordance with claim 5, further comprising a means to flow said analyte fluid from said inlet port to said fluid concentrator and to said sensor array.

7. A device in accordance with claim 1, wherein said sample chamber comprises an internally controlled atmosphere.

8. A device in accordance with claim 1, wherein said absorbent material is a member selected from the group consisting of a nanoporous material, a microporous material, a nonporous material and combinations thereof.

9. A device in accordance with claim 8, wherein said absorbent material is a member selected from the group consisting of activated carbon, silica gel, activated alumina, molecular sieve carbon, molecular sieve zeolites, silicalite, AlPO₄, a polymer, a co-polymer, alumina and mixtures thereof.

10. A device in accordance with claim 8, wherein said absorbent has a pore size from about 1 nm to about 100 nm.

11. A device in accordance with claim 10, wherein said absorbent has a pore size from about 1 nm to about 50 nm.

12. A device in accordance with claim 1, wherein said concentrated analyte is desorbed using a member selected from the group consisting of heating, purging, stripping, pressuring or a combination thereof.

13. A device in accordance with claim 1, further comprising an additional adsorbent to remove unwanted material from said analyte fluid.

14. A device in accordance with claim 13, wherein said unwanted material is a gas contaminate.

15. A device in accordance with claim 13, wherein said unwanted material is moisture.

16. A device in accordance with claim 1, wherein said response is an electrical response, and wherein said electrical response is a member selected from the group consisting of electrical resistance, impedance and capacitance.

17. A device in accordance with claim 1, wherein said array of sensors comprises a member selected from the group consisting of a surface acoustic wave sensor, a quartz microbalance sensor;

a conductive composite;

a chemiresistor;

a metal oxide gas sensor and a conducting polymer sensor, a dye-impregnated polymer film on fiber optic detector, a polymer-coated micromirror, an electrochemical gas detector, a chemically sensitive field-effect transistor, a carbon black-polymer composite, a micro-electro-mechanical system device and a micro-opto-electro-mechanical system device.

18. A device in accordance with claim 1, wherein said analyte is a member selected from the group consisting of alkanes, alkenes, alkynes, dienes, alicyclic hydrocarbons, arenes, alcohols, ethers, ketones, aldehydes, carbonyls, carbanions, polynuclear aromatics, heterocycles, organic derivatives, biomolecules, microorganisms, fingi, bacteria, microbes, viruses, metabolites, sugars, isoprenes and isoprenoids, fatty acids and their derivatives.

19. A device in accordance with claim 1, wherein said device is used in an application selected from the group consisting of environmental toxicology, remediation, biomedicine, material quality control, food monitoring, agricultural monitoring, heavy industrial manufacturing, ambient air monitoring, worker protection, emissions control, product quality testing, oil/gas petrochemical applications, combustible gas detection, H₂S monitoring, hazardous leak detection, emergency response and law enforcement applications, explosives detection, utility and power applications, food/beverage/agriculture applications, freshness detection, fruit ripening control, fermentation process monitoring and control, flavor composition and identification, product quality and identification, refrigerant and fumigant detection, cosmetic/perfume applications, fragrance formulation, chemical/plastics/pharmaceuticals applications, fugitive emission identification, solvent recovery effectiveness, hospital/medical applications, anesthesia and sterilization gas detection, infectious disease detection, breath analysis and body fluids analysis.

20. A device in accordance with claim 1, further comprising robotic armature for high throughput assay screening.

21. A device in accordance with claim 1, wherein said array of sensors comprise about 10 to about 100 sensors.

22. A device in accordance with claim 1, wherein said array of sensors comprise about 100 to about 1000 sensors.

23. A device in accordance with claim 1, wherein said array of sensors comprise about 1000 to about 10⁶ sensors.

24. A device in accordance with claim 1, wherein at least two sensors are compositionally different.

25. A device in accordance with claim 1, further comprising a computer, response profiles and an algorithm.

26. A device in accordance with claim 25, wherein said algorithm is a pattern recognition algorithm.

27. A device in accordance with claim 25, further comprising a neural network for software or hardware analysis.

28. A device in accordance with claim 1, wherein said device is a handheld device.

29. The device in accordance with claim 1, wherein said respiratory tract infection is a lower respiratory tract infection.

30. The device in accordance with claim 1, wherein said respiratory tract infection is an upper respiratory tract infection.

31. The device in accordance with claim 29, wherein said lower respiratory tract infection is selected from pneumonia or bronchitis.

32. The device in accordance with claim 30, wherein said upper respiratory tract infection is selected from sinusitis or pharyngitis.

33. A device for detecting a respiratory tract infection by detecting an indicative marker gas, said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said marker gas;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said marker gas and capable of desorbing a concentrated marker gas; and

c) an array of sensors in fluid communication with said concentrated marker gas.

34. The device in accordance with claim 33, wherein said respiratory tract infection is a lower respiratory tract infection.

35. The device in accordance with claim 33, wherein said respiratory tract infection is an upper respiratory tract infection.

36. The device in accordance with claim 34, wherein said lower respiratory tract infection is selected from pneumonia or bronchitis.

37. The device in accordance with claim 34, wherein said upper respiratory tract infection is selected from sinusitis or pharyngitis.

38. A device for detecting a urinary tract infection by detecting an indicative marker gas, said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said indicative marker gas;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said indicative marker gas and capable of desorbing a concentrated indicative marker gas; and

c) an array of sensors in fluid communication with said concentrated indicative marker gas.

39. The device in accordance with claim 38, wherein said indicative marker gas is a microorganism off gas selected from Enterobacteriaceae, Enterococcus sp. and combinations thereof.

40. A device for detecting bacterial vaginitis by detecting an indicative marker gas, said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said indicative marker gas;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said indicative marker gas and capable of desorbing a concentrated indicative marker gas; and

c) an array of sensors in fluid communication with said concentrated indicative marker gas.

41. The device in accordance with claim 40, wherein said indicative marker gas is selected from the group consisting of methylamine, isobutylamine, putrescine, cadaverine, histamine, tyramine, and phenethylamine.

42. A device for detecting the presence of an analyte, wherein said analyte is a microorganism marker gas indicative of a urinary tract infection said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said indicative marker gas;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said indicative marker gas and capable of desorbing a concentrated indicative marker gas; and

c) an array of sensors in fluid communication with said concentrated indicative marker gas.

43. The device in accordance with claim 42, wherein said indicative marker gas is an off gas from Enterobacteriaceae, Enterococcus sp. and combinations thereof.

44. A device for detecting the presence of an analyte, wherein said analyte is a marker gas indicative of bacterial vaginitis, said device comprising:

a) a sample chamber having a fluid inlet port for the influx of said indicative marker gas;

b) a fluid concentrator in flow communication with said sample chamber, said fluid concentrator having an absorbent material capable of absorbing said indicative marker gas and capable of desorbing a concentrated indicative marker gas; and

c) an array of sensors in fluid communication with said concentrated indicative marker gas.

45. The device in accordance with claim 44, wherein said indicative marker gas is selected from the group consisting of methylamine, isobutylamine, putrescine, cadaverine, histamine, tyramine, and phenethylamine.