Method for assaying L-phenylalanine and L-phenylalanine sensor
First Claim
1. A method of determining L-phenylalanine comprising:
- (1) adding a sample to an L-phenylalanine sensor composed of an electrode system which is formed by printing with the use of a conductive material and which comprises at least a working electrode made of a material mainly comprising carbon and a counter electrode formed on an insulating support, and a reagent reaction layer which is provided in a space of said electrode system comprising a working electrode and a counter electrode formed opposite to each other, and the reagent reaction layer contains as reagents at least L-phenylalanine dehydrogenase, NAD+ an or NADP+ as a coenzyme and phenazine as an electron mediator and which is fixed within said electrode system, and (2) electrochemically quantifying L-phenylalanine.
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Abstract
An L-phenylalanine sensor wherein all of the reagents required in determining L-phenylalanine are integrated together, thereby enabling quick and convenient quantification without resort to any special equipment, devices or techniques. This sensor is fabricated by integrating L-phenylalanine dehydrogenase, oxidized nicotinamide adenine dinucleotide (NAD+) or oxidized nicotinamide adenine dinucleotide phosphate (NADP+) as a coenzyme and an electron mediator, which are employed as reagents, with an electrode system comprising at least a working electrode and a counter electrode. Also, a method of determining L-phenylalanine which comprises adding a sample containing L-phenylalanine to the above-described sensor and electrochemically quantitating L-phenylalanine is provided.
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Citations
3 Claims
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1. A method of determining L-phenylalanine comprising:
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(1) adding a sample to an L-phenylalanine sensor composed of an electrode system which is formed by printing with the use of a conductive material and which comprises at least a working electrode made of a material mainly comprising carbon and a counter electrode formed on an insulating support, and a reagent reaction layer which is provided in a space of said electrode system comprising a working electrode and a counter electrode formed opposite to each other, and the reagent reaction layer contains as reagents at least L-phenylalanine dehydrogenase, NAD+ an or NADP+ as a coenzyme and phenazine as an electron mediator and which is fixed within said electrode system, and (2) electrochemically quantifying L-phenylalanine.
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- 2. An L-phenylalanine sensor having a structure composed of an electrode system which is formed by printing with the use of a conductive material and which comprises at least a working electrode made of a material mainly comprising carbon and a counter electrode formed on an insulating support, and a reagent reaction layer which is provided in a space of said electrode system comprising a working electrode and a counter electrode formed opposite to each other, and the reagent reaction layer contains as reagents at least L-phenylalanine dehydrogenase, NAD+ or NADP+ as a coenzyme and phenazine as an electron mediator and which is fixed within said electrode system.
Specification