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Method for assaying L-phenylalanine and L-phenylalanine sensor

  • US 6,468,416 B1
  • Filed: 01/12/2001
  • Issued: 10/22/2002
  • Est. Priority Date: 07/16/1998
  • Status: Expired due to Fees
First Claim
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1. A method of determining L-phenylalanine comprising:

  • (1) adding a sample to an L-phenylalanine sensor composed of an electrode system which is formed by printing with the use of a conductive material and which comprises at least a working electrode made of a material mainly comprising carbon and a counter electrode formed on an insulating support, and a reagent reaction layer which is provided in a space of said electrode system comprising a working electrode and a counter electrode formed opposite to each other, and the reagent reaction layer contains as reagents at least L-phenylalanine dehydrogenase, NAD+ an or NADP+ as a coenzyme and phenazine as an electron mediator and which is fixed within said electrode system, and (2) electrochemically quantifying L-phenylalanine.

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