Electrochemiluminescent rhenium moieties

• US 6,468,741 B1
• Filed: 09/21/1998
• Issued: 10/22/2002
• Est. Priority Date: 04/30/1986
• Status: Expired due to Fees

• Current Assignee: BioVeris Corporation (Roche Holding AG)
• Original Assignee: IGEN International, Inc. (Roche Holding AG)
• Inventors: Ciana, Leopoldo Della, Leland, Jonathan K., Dressick, Walter J., Poonian, Mohindar S., Hino, Janel K., Powell, Michael J., Massey, Richard J.
• Primary Examiner(s): Marschel, Ardin H.
• Application Number:
  US09/157,788
• Time in Patent Office: 1,492 Days
• Field of Search: 435/6, 435/7.1, 436/501, 536/23.1, 536/25.3, 935/77.78, 530/300, 530/333, 530/350
• US Class Current: 435/6.13
• CPC Class Codes: C07D 213/22 containing two or more pyridine rings directly linked together, e.g. bipyridyl
• CPC Class Codes: C07D 213/48 Aldehydo radicals
• CPC Class Codes: C07D 213/55 Acids; Esters
• CPC Class Codes: C07D 401/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: C07D 405/14 containing three or more hetero rings
• CPC Class Codes: C07D 473/08 with methyl radicals in positions 1 and 3, e.g. theophylline
• CPC Class Codes: C07F 13/00 Compounds containing elements of Groups 7 or 17 of the Periodic Table
• CPC Class Codes: C07F 15/0026 without a metal-carbon linkage
• CPC Class Codes: C07F 15/0053 without a metal-carbon linkage
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 2458/30 Electrochemically active labels
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/76 Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A labeled substance comprising a biological substance linked to a luminescent rhenium-containing label. Qualitative and quantitative electrochemiluminescent assays using the same. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

Claims

1. A labeled substance having the formula

2. The labeled substance of claim 1, wherein said biological substance is selected from the group consisting of whole cells, subcellular particles, polypeptides, nucleic acids, polysaccharides, alkaloids, steroids, vitamins, and amino acids.

3. The labeled substance of claim 1, wherein said biological substance is an antibody or a nucleic acid.

4. The labeled substance of claim 1, wherein t is greater than 1.

5. The labeled substance of claim 4, wherein said biological substance is selected from the group consisting of whole cells, subcellular particles, polypeptides, nucleic acids, polysaccharides, alkaloids, steroids, vitamins, and amino acids.

6. The labeled substance of claim 1, wherein B is covalently linked to P.

7. The labeled substance of claim 6, wherein said biological substance is selected from the group consisting of whole cells, subcellular particles, polypeptides, nucleic acids, polysaccharides, alkaloids, steroids, vitamins, and amino acids.

8. The labeled substance of claim 1, wherein L² is pyridine or substituted pyridine.

9. The labeled substance of claim 8, wherein said biological substance is selected from the group consisting of whole cells, subcellular particles, polypeptides, nucleic acids, polysaccharides, alkaloids, steroids, vitamins, and amino acids.

10. The labeled substance of claim 1, wherein B is covalently linked to L².

11. The labeled substance of claim 10, wherein said biological substance is selected from the group consisting of whole cells, subcellular particles, polypeptides, nucleic acids, polysaccharides, alkaloids, steroids, vitamins, and amino acids.

12. A label having the formula

13. The label of claim 12, wherein W is —

CO₂Su.

14. The label of claim 12, wherein P has no substituents.

15. The label of claim 14, wherein W is —

CO₂Su.

16. A label subastance comprising a biological substance linked to a luminescent coordinate complex of rhenium, wherein said luminescent coordinate complex is a rhenium tricarbonyl complex.