Agent and method for detecting chemical substances
First Claim
1. A method for the detection of chemical substances, comprising:
- providing an agent comprising a first polynucleotide or peptide sequence having a first fluorophoric molecule, wherein first end of said first polynucleotide or peptide sequence is bonded to a solid phase, and a second polynucleotide or peptide sequence having a second fluorophoric molecule, wherein a second end of said second polynucleotide or peptide sequence has a group which is sensitive to the chemical substance to be detected, said chemical substance comprising a physical property sensitive to an external force, the first polynucleotide or peptide sequence being hybridizable to the second polynucleotide or peptide sequence such that a spatial relationship making possible an interaction between the first and the second fluorophoric molecule is producible, and the spatial relationship being terminatable and thus a fluorescence reaction being producible upon addition of the chemical substance to the group and action of an external force;
contacting the agent with the chemical substance to be detected;
applying the external force; and
detecting the presence or absence of the fluorescence reaction.
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Accused Products
Abstract
The invention relates to an agent for detecting chemical substances, comprising: a first polynucleotide or peptide sequence having a first fluorophore molecule, the first end of said sequence being bonded on a solid phase and a second polynucleotide or peptide sequence having a second fluorophore molecule, the second end of said sequence having a group that is sensitive to the chemical substance to be detected. The first polynucleotide or peptide sequence can be hybridized to the second polynucleotide or peptide sequence in such a way that a spatial relationship enabling an interaction betweeen the first and the second fluorophore molecule is produced. When the chemical substance including a physical property is attached to the group and the influence of an external force is exerted on the physical property, the spatial relation can be eliminated, thereby generating or abolishing a fluorescent reaction.
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Citations
21 Claims
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1. A method for the detection of chemical substances, comprising:
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providing an agent comprising a first polynucleotide or peptide sequence having a first fluorophoric molecule, wherein first end of said first polynucleotide or peptide sequence is bonded to a solid phase, and a second polynucleotide or peptide sequence having a second fluorophoric molecule, wherein a second end of said second polynucleotide or peptide sequence has a group which is sensitive to the chemical substance to be detected, said chemical substance comprising a physical property sensitive to an external force, the first polynucleotide or peptide sequence being hybridizable to the second polynucleotide or peptide sequence such that a spatial relationship making possible an interaction between the first and the second fluorophoric molecule is producible, and the spatial relationship being terminatable and thus a fluorescence reaction being producible upon addition of the chemical substance to the group and action of an external force;
contacting the agent with the chemical substance to be detected;
applying the external force; and
detecting the presence or absence of the fluorescence reaction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. An agent for the detection of chemical substances, having a first polynucleotide or peptide sequence having a first fluorophoric molecule, wherein a first end of said first polynucleotide or peptide sequence is bonded to a solid phase, and a second polynucleotide or peptide sequence having a second fluorophoric molecule, wherein a second end of said second polynucleotide or peptide sequence has a group which is sensitive to the chemical substance to be detected, said chemical substance comprising a physical property sensitive to an external force, the first polynucleotide or peptide sequence being hybridizable to the second polynucleotide or peptide sequence such that a spatial relationship making possible an interaction between the first and the second fluorophoric molecule is producible, and the spatial relationship being terminatable and thus a fluorescence reaction being producible in the presence of the chemical substance and action of said external force, wherein the spatial relationship is designed in the form of a secondary structure, wherein the secondary structure is a hairpin loop, a helix or a pleated sheet.
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21. An agent for the detection of chemical substances, having a first polynucleotide or peptide sequence having a first fluorophoric molecule, wherein a first end of said first polynucleotide or peptide sequence is bonded to a solid phase, and a second polynucleotide or peptide sequence having a second fluorophoric molecule, wherein a second end of said second polynucleotide or peptide sequence has a group which is sensitive to the chemical substance to be detected, said chemical substance comprising a physical property sensitive to an external force, the first polynucleotide or peptide sequence being hybridizable to the second polynucleotide or peptide sequence such that a spatial relationship making possible an interaction between the first and the second fluorophoric molecule is producible, and the spatial relationship being terminatable and thus a fluorescence reaction being producible in the presence of the chemical substance and action of said external force, wherein the solid phase is plastic, wherein the plastic is electrically conductive.
Specification