Biosensor

• US 6,471,839 B1
• Filed: 05/17/2000
• Issued: 10/29/2002
• Est. Priority Date: 05/20/1999
• Status: Expired due to Fees

• Current Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Yamamoto, Tomohiro, Yoshioka, Toshihiko, Nankai, Shiro
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/572,797
• Time in Patent Office: 895 Days
• Field of Search: 204/403, 204/403.06, 204/403.1
• US Class Current: 204/403.06
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence

Abstract

The present invention provides a biosensor which enables quick measurement and is excellent in storage characteristic. This biosensor comprises an electrically insulating base plate on which an electrode system having at least a measuring electrode and a counter electrode is formed, a cover member which is integrated to the base plate so as to form a sample solution supply path for supplying a sample solution to the electrode system between the cover member and the base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, and the carrier is placed in the sample solution supply path. Preferably, the carrier is constituted at least of two carrier pieces and each carrier piece supports a different reagent.

Claims

1. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, in the vicinity of, but not contacting, the electrode system.

2. The biosensor in accordance with claim 1, wherein said carrier is fixed in said sample solution supply path by an adhesive.

3. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is placed in said sample solution supply path and wherein said carrier is fixed in said sample solution supply path by an adhesive, further wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator and said adhesive is a surfactant.

4. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.

5. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent comprises at least cholesterol oxidase, cholesterol esterase, a surfactant and an electron mediator, and the carrier piece containing the cholesterol esterase contains the surfactant.

6. The biosensor in accordance with claim 5, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase, a surfactant and an electron mediator, and wherein said electrode system comprises silver and a carbon layer covering the silver, and said electrode system is coated with a hydrophilic polymer.

7. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, the carrier comprising at least two carrier pieces, each carrier piece supporting a different reagent, wherein said carrier is fixed by an adhesive in the vicinity of, but not contacting, said electrode system.

8. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and said adhesive is a surfactant.

9. The biosensor in accordance with claim 8, wherein said electrode system comprises silver and a,carbon layer covering the silver, and said electrode system is coated with a hydrophilic polymer.

10. A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.