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Hybrid matrix implants and explants

  • US 6,472,181 B1
  • Filed: 05/14/1999
  • Issued: 10/29/2002
  • Est. Priority Date: 10/25/1995
  • Status: Expired due to Fees
First Claim
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1. A method of producing a polypeptide in vitro, comprising:

  • providing a composition comprising a body of matrix material comprising insoluble collagen fibrils, there being embedded within the body of matrix material (a) a plurality of cultured vertebrate cells which express the polypeptide and (b) a plurality of microspheres;

    maintaining the composition under conditions whereby the cultured vertebrate cells express and secrete the polypeptide;

    contacting the composition with a liquid such that the cells secrete the polypeptide into the liquid; and

    obtaining the polypeptide from the liquid.

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