Comparative phenotype analysis
First Claim
1. A testing system for measuring at least 95 nutritional phenotypes of at least one cell type, comprising a testing device having a plurality of testing wells wherein said testing wells contain at least one test substrate selected from the group consisting of nitrogen sources selected from the group consisting of nitrogen-containing compounds, phosphorus sources selected from the group consisting of phosphorus-containing compounds, sulfur sources selected from the group consisting of sulfur-containing compounds, and auxotrophic supplements selected from the group consisting of amino acids, vitamins, polyamines, lactones, fatty acids, nucleic acid bases, nucleosides and nucleotides;
- inoculating fluid comprising a gelling agent, under conditions such that said gelling agent remains ungelled; and
an instrument capable of incubating and recording the response of said at least one cell type placed in said testing device.
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Abstract
The present invention relates to growing and testing microorganisms in a multitest format which utilizes a gel forming matrix for the rapid screening of clinical and environmental cultures. The present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments (e.g., the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi). The present invention is also particularly suited for comparative analysis of phenotypic differences between cell types, including strains of microorganisms that have been designated as the same genus and species, as well as other cell types (e.g., mammalian, insect, and plant cells).
27 Citations
24 Claims
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1. A testing system for measuring at least 95 nutritional phenotypes of at least one cell type, comprising a testing device having a plurality of testing wells wherein said testing wells contain at least one test substrate selected from the group consisting of nitrogen sources selected from the group consisting of nitrogen-containing compounds, phosphorus sources selected from the group consisting of phosphorus-containing compounds, sulfur sources selected from the group consisting of sulfur-containing compounds, and auxotrophic supplements selected from the group consisting of amino acids, vitamins, polyamines, lactones, fatty acids, nucleic acid bases, nucleosides and nucleotides;
- inoculating fluid comprising a gelling agent, under conditions such that said gelling agent remains ungelled; and
an instrument capable of incubating and recording the response of said at least one cell type placed in said testing device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
- inoculating fluid comprising a gelling agent, under conditions such that said gelling agent remains ungelled; and
Specification