Assay using porosity-reduction to inhibit migration
First Claim
1. A device for assaying spermatozoa, comprising:
- a labeling zone containing at least one label which binds to the spermatozoa in their presence and through which spermatozoa can flow in communication with;
a porous capture zone having a pore size such that all or substantially all of the label which is not bound to the spermatozoa can flow therethrough, whereas all or substantially all of the spermatozoa-bound label cannot.
1 Assignment
0 Petitions
Accused Products
Abstract
Device for assaying an analyte, comprising a labelling zone, where a label can bind to the analyte, in communication with a capture zone, wherein the pore size of the capture zone is such that label which is not bound to the analyte can migrate therethrough, whereas label which is bound to the analyte cannot. During migration from the labelling zone (large pore size) to the capture zone (small pore size), unbound label can pass into and through the capture zone, whereas bound label will be captured at the junction of the labelling zone a the capture zone. The device relies upon the label being smaller than the analyte, such that free label is not retarded by the capture zone. It is particularly suitable for assying analytes such as spermatozoa, which are large in comparison with a label such as a labelled antibody.
-
Citations
13 Claims
-
1. A device for assaying spermatozoa, comprising:
-
a labeling zone containing at least one label which binds to the spermatozoa in their presence and through which spermatozoa can flow in communication with;
a porous capture zone having a pore size such that all or substantially all of the label which is not bound to the spermatozoa can flow therethrough, whereas all or substantially all of the spermatozoa-bound label cannot. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
Specification