Imaging and analyzing parameters of small moving objects such as cells

• US 6,473,176 B2
• Filed: 03/29/2001
• Issued: 10/29/2002
• Est. Priority Date: 01/25/1999
• Status: Expired due to Term

• Current Assignee: Cytek Biosciences Inc
• Original Assignee: Amnis Corporation (DiaSorin S.p.A.)
• Inventors: Basiji, David A., Ortyn, William E.
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US09/820,434
• Publication Number:
  US 20010021018A1
• Time in Patent Office: 579 Days
• Field of Search: 356/416, 356/417, 356/419, 359/633, 359/634, 348/336, 348/338, 348/340, 358/515, 250/226
• US Class Current: 356/326
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01J 3/2823 Imaging spectrometer
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0294 Particle shape
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1472 with colour
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2021/052 Tubular type; cavity type; multireflective
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G02B 27/1013 for colour or multispectral image sensors, e.g. splitting an image into monochromatic image components on respective sensors spectral imaging systems G01J
• CPC Class Codes: G02B 27/126 The splitting element being a prism or prismatic array, including systems based on total internal reflection
• CPC Class Codes: G02B 27/144 using partially transparent surfaces without spectral selectivity G02B27/147 takes precedence
• CPC Class Codes: G02B 27/145 having sequential partially reflecting surfaces
• CPC Class Codes: G02B 27/148 including stacked surfaces having at least one double-pass partially reflecting surface
• CPC Class Codes: G02B 7/28 Systems for automatic generation of focusing signals

Abstract

Light from an object such as a cell moving through an imaging system is collected and dispersed so that it can be imaged onto a detector. The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system can be imaged, producing both scatter images and dispersed images at different locations on one or more detectors. In one embodiment, the detector is divided into a plurality of zones so that each of a plurality of different images are spectrally dispersed onto a different zone of the detector.

Claims

1. An imaging system adapted to determine one or more characteristics of an object from an image of the object, comprising:

(a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path;

(b) a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time; and

(c) a detector disposed to receive light that has been reflected by the light reflecting elements, producing an output signal that is indicative of at least one characteristic of the object, said detector integrating the output signal over time.

2. The imaging system of claim 1, wherein wedge-shaped substrates define an angular difference between each light reflecting element, and wherein light reflecting surfaces of the light reflecting elements are sandwiched between the wedge-shaped substrates, forming a monolithic structure.

3. The imaging system of claim 1, where the light reflecting elements reflect light based on a spectral content of that light.

4. The imaging system of claim 1, where the light reflecting elements reflect light as a function of a polarization characteristic of the light.

5. The Imaging system of claim 1, wherein the collection lens substantially collimates the light that is traveling along the optical path toward a plurality of light reflective elements.

6. The imaging system of claim 1, further comprising an imaging lens disposed to receive light reflected from the plurality of reflective elements, said imaging lens producing an image of the light on the detector.

7. The imaging system of claim 1, wherein the light traveling in each different direction from each of the plurality of reflecting elements is incident on a different region of the detector.

8. The imaging system of claim 1, wherein the detector comprises a time delay and integration (TDI) detector.

9. The imaging system of claim 1, further comprising a filter disposed between the plurality of light reflective elements and the detector, said filter attenuating light that does not have the predefined characteristic of the light reflected from at least one of the plurality of light reflecting elements.

10. The imaging system of claim 9, wherein the filter comprises a plurality of spectral regions, each having a different one of the predefined characteristics of the plurality of light reflecting elements so that the light reflected from each of the plurality of light reflecting elements associated with each predefined characteristic is incident on a corresponding spectral region of the filter, said spectral region passing the light having the predefined characteristic and blocking light that does not have the predefined characteristic of said spectral region.

11. The imaging system of claim 1, wherein said detector comprises a pixelated detector.

12. A method for determining one or more characteristics of an object based upon light from the object, comprising the steps of:

(a) focussing the light from the object along a collection path;

(b) at each of a plurality of successive points disposed along the collection path, reflecting light of a predefined characteristic, and passing light that does not have the predefined characteristic, a different predefined characteristic being associated with each of the plurality of points so that light of a different one of the different predefined characteristics is reflected from each successive point in a direction different from that at other points, and so that light reflected at all but a first point of the plurality of successive points passes through at least one preceding point of the successive points a second time;

(c) receiving the light that was reflected at a detector, said detector integrating an output signal over time in response thereto; and

(d) analyzing the output signal from the detector to determine at least one characteristic of the object.

13. The method of claim 12, wherein each of the plurality of light reflecting elements associated with a specific different one of the different characteristics is disposed at each successive point, further comprising the step of joining the plurality of light reflecting elements together with intermediate wedge-shaped substrates, forming a monolithic structure that disperses the light in the different directions as a function of the different predefined characteristics associated with the plurality of light reflecting elements.

14. The method of claim 12, wherein the step of reflecting light in different directions comprises the step of reflecting the light in a specific direction based on a spectral content of the light that is thus reflected.

15. The method of claim 12, wherein the light traveling along the collection path has a plurality of different polarization characteristics, said step of reflecting comprising the step of reflecting the light in a specific direction based upon a polarization characteristic of the light that is thus reflected.

16. The method of claim 12, further comprising the step of imaging the light traveling in each different direction using a lens.

17. The method of claim 12, further comprising the step of producing a plurality of signals, each signal corresponding to an intensity of the light traveling in one of the different directions and with one of the plurality of different predefined characteristics.

18. The method of claim 12, wherein the step of detecting includes the step of providing a time delay and integration (TDI) detector for detecting the light that is reflected.

19. The method of claim 12, wherein the step of detecting further includes the step of detecting the intensity of the light traveling in each different direction on each of a plurality of spaced-apart regions on the detector.

20. The method of claim 12, further comprising the step of attenuating light reflected from each successive point that does not have the predefined characteristic associated with the successive point in the optical path from which the light was reflected.

21. The method of claim 20, wherein the step of attenuating comprises the step of filtering so that only light having the predefined characteristic associated with the successive point from which the light was reflected is allowed to pass to the detector.

22. The method of claim 12, wherein the step of focussing comprises the step of collimating the light traveling along the optical path prior to said light reaching the plurality of successive points.

23. An imaging system adapted to determine one or more characteristics of an object from an image of the object while there is relative movement between the object and the imaging system, comprising:

(a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path;

(b) a dispersing component comprising a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time;

(c) an imaging lens disposed to receive the light from the dispersing component, producing a plurality of images, each image corresponding to a different one of the directions in which light is reflected by the plurality of light reflecting elements, each image being projected by the imaging lens toward a different predetermined location; and

(d) a detector disposed to receive the plurality of images produced by the imaging lens as the images move over a surface of the detector due to the relative movement between the object and the imaging system, producing an output that is indicative of at least one characteristic of the object, while the relative movement between the object and the imaging system occurs.

24. The imaging system of claim 23, wherein the plurality of light reflecting elements comprises a plurality of beam splitters arranged to reflect light of predetermined spectral characteristics at different predefined angles, all light from each beam splitter corresponding to a separate light beam, each light beam leaving the spectral dispersing component at a different nominal angle.

25. The imaging system of claim 24, wherein wedge-shaped substrates define an angular difference between each of the plurality of beam splitters, and wherein the plurality of beam splitters are sandwiched between the wedge-shaped substrates, forming a monolithic structure.

26. The imaging system of claim 23, wherein the dispersing component disperses light in a direction substantially orthogonal to a direction of the relative movement between the object and the imaging system.

27. The imaging system of claim 23, wherein the dispersing component disperses light in a direction substantially aligned with a direction of the relative movement between the object and the imaging system.

28. The imaging system of claim 23, wherein the detector comprises a time delay and integration (TDI) detector that is divided into a plurality of segments, each segment receiving light from a different one of the plurality of light reflecting elements.

29. The imaging system of claim 23, wherein the detector includes a plurality of taps, a signal indicative of different ones of the plurality of images received by the detector being output from corresponding different ones of the plurality of taps.

30. The imaging system of claim 29, wherein the detector has a length and a width, said width being divided into a plurality of regions, each region having a corresponding one of the plurality of taps, a charge generated in each region in response to the region receiving one of the plurality of images being integrated over the length of the detector.

31. The imaging system of claim 30, wherein a readout rate from each tap is independently controllable.

32. The imaging system of claim 23, wherein the light from the object comprises at least one of an unstimulated emission from the object, light reflected by the object, scattered light from the object, and a stimulated emission from the object.

33. The imaging system of claim 23, further comprising a light source that is disposed to provide an incident light that illuminates the object.

34. The imaging system of claim 33, wherein the light source illuminates the object with a bright field.

35. The imaging system of claim 33, further comprising a substrate on which the object is disposed.

36. An imaging system adapted to determine one or more characteristics of an object from an image of the object, comprising:

(a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path;

(b) a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time; and

(c) a time delay and integration (TDI) detector disposed to receive light that has been reflected by the light reflecting elements, producing an output signal that is indicative of at least one characteristic of the object, said detector integrating the output signal over time in response to the light from the light reflecting elements.

37. A method for determining one or more characteristics of an object based upon light from the object, comprising the steps of:

(a) focussing the light from the object along a collection path;

(b) at each of a plurality of successive points disposed along the collection path, reflecting light of a predefined characteristic, and passing light that does not have the predefined characteristic, a different predefined characteristic being associated with each of the plurality of points so that light of a different one of the different predefined characteristics is reflected from each successive point in a direction different from that at other points, and so that light reflected at all but a first point of the plurality of successive points passes through at least one preceding point of the successive points a second time;

(c) receiving the light that was reflected at a detector, said detector including a plurality of taps and having a length and a width, said width being divided into a plurality of regions, each region having a corresponding one of the plurality of taps, a charge generated in each region in response to the region receiving light from one of the points being integrated over the length of the detector to produce an output signal; and

(d) analyzing the output signal from at least one of the plurality of taps of the detector to determine at least one characteristic of the object.

38. An imaging system adapted to determine one or more characteristics of an object from an image of the object while there is relative movement between the object and the imaging system, comprising:

(a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path;

(b) a dispersing component comprising a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time;

(c) an imaging lens disposed to receive the light from the dispersing component, producing a plurality of images, each image corresponding to a different one of the directions in which light is reflected by the plurality of light reflecting elements, each image being projected by the imaging lens toward a different predetermined location; and

(d) a detector disposed to receive the plurality of images produced by the imaging lens, said detector including a plurality of taps and having a length and a width, said width being divided into a plurality of regions, each region having a corresponding one of the plurality of taps, a charge generated in each region in response to the region receiving light for one of the images being integrated over the length of the detector to produce an output signal at one of the plurality of taps that is indicative of at least one characteristic of the object.

39. An imaging system adapted to determine one or more characteristics of an object in a broad, flat flow, from an image of the object, comprising:

(a) a collection lens having a field angle in an object space that is sufficiently large to collect light traveling from the object that is in the broad, flat flow so that the light passes through the collection lens and travels along a collection path;

(b) a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time; and

(c) a detector disposed to receive light that has been reflected by the light reflecting elements, producing an output signal that is indicative of at least one characteristic of the object, said detector integrating the output signal over time.

40. An imaging system adapted to determine one or more characteristics of an object in a broad, flat flow, from an image of the object while there is relative movement between the object and the imaging system comprising:

(a) a collection lens having a field angle in an object space that is sufficiently large to collect light traveling from the object that is in the broad, flat flow so that the light passes through the collection lens and travels along a collection path;

(b) a plurality of light reflecting elements disposed in the collection path, each light reflecting element reflecting light of a predefined characteristic and passing light that does not have that characteristic, each light reflecting element being positioned at a different angle with respect to the collection path to reflect light of the predefined characteristic in a direction different from that of other light reflecting elements, each light reflecting element being positioned adjacent to a preceding light reflecting element such that light reflected by all but a first light reflecting element passes through at least one preceding light reflecting element a second time;

(c) an imaging lens disposed to receive the light beams from the dispersing component, producing a plurality of images corresponding to each of the light beams, each image being projected by the imaging lens toward a different predetermined location; and

(d) a time delay integration (TDI) detector disposed to receive the plurality of images produced by the imaging lens, producing an output signal that is indicative of at least one characteristic of the object in the broad, flat flow, said TDI detector producing the output signal by integrating light from at least a portion of the object over time, while the relative movement between the object and the imaging system occurs.