Total hemoglobin concentration measurement

• US 6,473,632 B1
• Filed: 06/16/2000
• Issued: 10/29/2002
• Est. Priority Date: 06/16/1999
• Status: Expired due to Term

• Current Assignee: Newmark, Inc., Hutchinson Technology Incorporated (TDK Corporation)
• Original Assignee: Hutchinson Technology Incorporated (TDK Corporation)
• Inventors: Myers, Dean E.
• Primary Examiner(s): Winakur, Eric F.
• Assistant Examiner(s): Kremer, Matthew
• Application Number:
  US09/595,148
• Time in Patent Office: 865 Days
• Field of Search: 600/309-311, 600/316, 600/322-324, 600/328, 600/331
• US Class Current: 600/322
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/7239 using differentiation including higher order derivatives
• CPC Class Codes: G01J 3/433 Modulation spectrometry; Derivative spectrometry
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3504 for analysing gases, e.g. multi-gas analysis

Abstract

A method for operating a spectrophotometric instrument of the type for measuring the oxygenation state of hemoglobin in tissue. The method includes the use of stored hemoglobin concentration relationship data characterizing the relationship between second derivative absorbance values at a hemoglobin-absorbing wavelength and hemoglobin concentration in a tissue as a function of hemoglobin oxygenation state. Data representative of a second derivative absorbance value of tissue being analyzed is received. The hemoglobin oxygenation state of the tissue is determined as a function of the second derivative absorbance value. The hemoglobin concentration in the tissue is then determined as a function of the hemoglobin concentration relationship data, the second derivative absorbance value and the hemoglobin oxygenation state. The accuracy of the hemoglobin oxygenation state can be determined as a function of the hemoglobin concentration value.

Claims

1. A method for measuring total concentration of a chromophore having at least two functional states in tissue, including:

providing stored relationship data representative of lines or curves which characterize the relationship between second-derivative absorbance values at a first chromophore-absorbing wavelength and total concentration of the chromophore in tissue at constant functional state portion values;

receiving a first second-derivative absorbance value from a tissue being analyzed at the first chromophore-absorbing wavelength;

receiving functional state portion data representative of the value of the functional state portion of the chromophore in the tissue being analyzed; and

generating data representative of the total concentration of the chromophore in the tissue being analyzed as a function of the line or curve of constant functional state portion of the chromophore associated with the received functional state portion data and the received first second-derivative absorbance value.

2. The method of claim 1, wherein the chromophore is hemoglobin, and wherein:

providing the stored relationship data includes providing data representative of lines or curves of constant %StO2;

receiving functional state portion data includes receiving data representative of a %StO2 portion value; and

generating data representative of the total concentration of hemoglobin includes generating the data as a function of the line or curve of constant %StO2 associated with the received data representing the %StO2 portion value and the received first second-derivative absorbance value.

3. The method of claim 2, wherein the first chromophore-absorbing wavelength is 760 nm.

4. The method of claim 2, wherein the data representative of the %StO2 portion value includes a second second-derivative absorbance value at a second chromophore-absorbing wavelength.

5. The method of claim 4, wherein the data representative of the %StO2 portion value includes a ratio of the second second-derivative absorbance value over the first second-derivative absorbance value.

6. The method of claim 4, wherein the second chromophore-absorbing wavelength is 720 nm.

7. The method of claim 6, wherein the first and second second-derivative absorbance values are calculated from absorbance values measured at each of 680 nm, 720 nm, 760 nm, and 800 nm.

8. The method of claim 2, and further including the step of:

determining validity of the %StO2 portion value as a function of the data representative of the total concentration of hemoglobin.

9. The method of claim 8, and further including the step of:

providing a display indicating the validity of the %StO2 portion value.

10. The method of claim 1 wherein the functional state portion data includes a second second-derivative absorbance value at a second chromophore-absorbing wavelength.

11. The method of claim 10, wherein the functional state portion data includes a ratio of the first second-derivative absorbance value and the second second-derivative absorbance value.

12. The method of claim 1, further including the step of:

determining validity of the functional state portion data as a function of the data representative of the total concentration of the chromophore.

13. The method of claim 12, further including the step of:

providing a display indicating the validity of the functional state portion data.

14. A spectrophotometic instrument for generating data representative of the total concentration of a chromophore in accordance with the method of claim 1.

15. The instrument of claim 14, configured to measure absorbance values at each of 680 nm, 720 nm, 760 nm and 800 nm.

16. A method for operating a spectrophotometric instrument of the type for measuring oxygenation state of hemoglobin in tissue, including:

providing stored hemoglobin concentration relationship data characterizing the relationship between second-derivative absorbance values at a first hemoglobin-absorbing wavelength and hemoglobin concentration in tissue as a function of hemoglobin oxygenation state;

receiving a first second-derivative absorbance value at the first hemoglobin-absorbing wavelength from a tissue being analyzed;

determining hemoglobin oxygenation state of the tissue being analyzed; and

determining the hemoglobin concentration in the tissue being analyzed as a function of the hemoglobin concentration relationship data, the first second-derivative absorbance value and the hemoglobin oxygenation state.

17. The method of claim 16, wherein the first hemoglobin-absorbing wavelength is 760 nm.

18. The method of claim 16, wherein determining the hemoglobin oxygenation state includes receiving a second second-derivative absorbance value from the tissue being analyzed at a second hemoglobin-absorbing wavelength.

19. The method of claim 18, wherein the second hemoglobin-absorbing wavelength is 720 nm.

20. The method of claim 18, wherein determining the hemoglobin oxygenation state includes calculating a ratio of the second second-derivative absorbance value over the first second-derivative absorbance value.