Bilateral microinjector pump for freely moving animals in an operant chamber

• US 6,475,188 B1
• Filed: 05/18/2000
• Issued: 11/05/2002
• Est. Priority Date: 05/20/1999
• Status: Expired due to Fees

• Current Assignee: Baxter, Anthony David
• Original Assignee: Baxter, Anthony David
• Inventors: Baxter, Anthony David
• Primary Examiner(s): Walberg, Teresa
• Assistant Examiner(s): Fastovsky, Leonid M
• Application Number:
  US09/573,591
• Time in Patent Office: 901 Days
• Field of Search: 604/67, 604/131, 604/185, 604/117, 604/132, 604/151, 604/154, 604/155, 604/156, 204/299.R, 435/285.1, 417/572, 222/325
• US Class Current: 604/131
• CPC Class Codes: A61M 2205/0244 Micromachined materials, e.g. made from silicon wafers, microelectromechanical systems [MEMS] or comprising nanotechnology
• CPC Class Codes: A61M 2210/0693 Brain, cerebrum
• CPC Class Codes: A61M 2250/00 Specially adapted for animals
• CPC Class Codes: A61M 5/1452 pressurised by means of pistons

Abstract

A microinjection pump for unrestrained and freely behaving animals is provided. The microinjection pump employs a screw drive mechanism that is actuated by an attached small stepper motor. The pump moves fluid by direct mechanical displacement from two microsyringes arranged in parallel (see FIGS. 1 and 2). The pump pivots and turns freely above an animal cage by an electrical swivel and connects to the animal head only by two small flexible capillary tubes that store and deliver fluids to both sides of its brain. Through associated electronic connections, the pump allows the animal to lever press for self-administration of fluids.

Claims

1. A recirculating micro ball bearing slide providing the frictionless mechanism for a movable block ( called a “

knee”

that is bolted to the ball bearing slide) and linear guide, providing the support and guide structure and housing for the knee and a stationary micro syringe holder and clamp;

knee has a machined tapped hole in it for receiving a threaded rod;

knee converts rotary motion of the turning threaded rod into its own linear motion along the guide rail;

machined key slots on the knee receive and hold the plunger heads of two bilaterally arranged micro syringes;

micro syringe clamp holds the barrels of the micro syringes stationary while the moving knee depresses the plungers in linear fashion causing extrusion of fluid from the micro syringes and into and through capillary tubing attached to the micro syringes for delivery of fluids into the freely behaving animal.

2. The microinjection pump, in accordance with claim 1, is actuated by a small stepper motor that is affixed to a flange that is itself bolted to the guide rail;

power for the stepper motor is delivered through a 4 channel electrical “

swivel”

commutator and modular socket connector that is wired to the stepper motor;

associated electronics give the motor step size of 15 degrees (24 steps per 360 degree revolution);

the stepper motor drive shaft is attached to the threaded rod by a bolt and turns it in a rotary and stepwise manner;

the threaded rod is screwed into the knee through the hole according to claim 1;

the knee, held by the micro recirculating ball bearing slide and, through friction with the turning threaded rod, moves linearly along the guide and depresses the plungers of the micro syringes, extruding fluid in a stepwise, precise and accurate method;

in reverse driving mode the stepper motor causes the knee to move in the opposite direction along the guide and retracts the micro syringe plungers and takes up fluid and fills the micro syringes.