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Arrays of proteins and methods of use thereof

  • US 6,475,808 B1
  • Filed: 07/14/1999
  • Issued: 11/05/2002
  • Est. Priority Date: 07/14/1998
  • Status: Expired due to Fees
First Claim
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1. An array device comprising:

  • a substrate defining a surface, and an array of discrete array-regions over the surface;

    an ordered hydrophobic-monolayer formed of alkyl-chains having alkyl-chain proximal ends which are chemisorbed or physisorbed to said surface, and opposite alkyl-chain distal ends;

    a hydrophilic-monolayer formed from hydrophilic-chains, each having a hydrophilic-chain proximal end by which each hydrophilic-chain is covalently linked to an alkyl-chain distal end, and an opposite hydrophilic-chain distal end, said ordered hydrophobic-monolayer and said hydrophilic-monolayer being effective in combination to resist non-specific protein binding; and

    , a plurality of protein-immobilizing groups covalently attached to a selected fraction of said hydrophilic-chains'"'"' distal ends within said array-regions, such that application of one or more selected proteins to selected array-regions is effective to form an array of protein-regions, each having a selected surface concentration of a selected protein carried in and displayed on said hydrophilic-monolayer, and separated from one another by border-regions effective to resist non-specific protein binding.

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