Arrays of proteins and methods of use thereof
First Claim
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1. An array device comprising:
- a substrate defining a surface, and an array of discrete array-regions over the surface;
an ordered hydrophobic-monolayer formed of alkyl-chains having alkyl-chain proximal ends which are chemisorbed or physisorbed to said surface, and opposite alkyl-chain distal ends;
a hydrophilic-monolayer formed from hydrophilic-chains, each having a hydrophilic-chain proximal end by which each hydrophilic-chain is covalently linked to an alkyl-chain distal end, and an opposite hydrophilic-chain distal end, said ordered hydrophobic-monolayer and said hydrophilic-monolayer being effective in combination to resist non-specific protein binding; and
, a plurality of protein-immobilizing groups covalently attached to a selected fraction of said hydrophilic-chains'"'"' distal ends within said array-regions, such that application of one or more selected proteins to selected array-regions is effective to form an array of protein-regions, each having a selected surface concentration of a selected protein carried in and displayed on said hydrophilic-monolayer, and separated from one another by border-regions effective to resist non-specific protein binding.
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Abstract
Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.
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Citations
3 Claims
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1. An array device comprising:
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a substrate defining a surface, and an array of discrete array-regions over the surface;
an ordered hydrophobic-monolayer formed of alkyl-chains having alkyl-chain proximal ends which are chemisorbed or physisorbed to said surface, and opposite alkyl-chain distal ends;
a hydrophilic-monolayer formed from hydrophilic-chains, each having a hydrophilic-chain proximal end by which each hydrophilic-chain is covalently linked to an alkyl-chain distal end, and an opposite hydrophilic-chain distal end, said ordered hydrophobic-monolayer and said hydrophilic-monolayer being effective in combination to resist non-specific protein binding; and
,a plurality of protein-immobilizing groups covalently attached to a selected fraction of said hydrophilic-chains'"'"' distal ends within said array-regions, such that application of one or more selected proteins to selected array-regions is effective to form an array of protein-regions, each having a selected surface concentration of a selected protein carried in and displayed on said hydrophilic-monolayer, and separated from one another by border-regions effective to resist non-specific protein binding. - View Dependent Claims (2, 3)
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Specification